Carbofuran accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish

Carbofuran is a carbamate pesticide, widely used in agricultural practices to increase crop productivity. In mammals, carbofuran is known to cause several untoward effects, such as apoptosis in the hippocampal neuron, oxidative stress, loss of memory and chromosomal anomalies. Most of these effects...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine 2021-01, Vol.25 (2), p.1048-1059
Main Authors: Khan, Alam, Fahad, Talukdar Mohammad, Akther, Tanjima, Zaman, Tanjeena, Hasan, Md Faruk, Islam Khan, Md Rafiqual, Islam, Mohammad Saiful, Kishi, Shuji
Format: Article
Language:English
Subjects:
Aging
Agricultural practices
Apoptosis
Autophagy
Carbofuran
Cell cycle
Danio rerio
Deoxyribonucleic acid
DNA
Embryos
Enzymes
Experiments
Fish
Hippocampus
life span
Lysosomes
Morphology
Mutants
Mutation
Nervous system
Original
Oxidative stress
Pesticides
Phagocytosis
Phenotypes
Plasmids
Potassium
RNA polymerase
Senescence
spinster homolog 1
Toxicity
Yolk
Zebrafish
β-Galactosidase
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Summary:Carbofuran is a carbamate pesticide, widely used in agricultural practices to increase crop productivity. In mammals, carbofuran is known to cause several untoward effects, such as apoptosis in the hippocampal neuron, oxidative stress, loss of memory and chromosomal anomalies. Most of these effects are implicated with cellular senescence. Therefore, the present study aimed to determine the effect of carbofuran on cellular senescence and biological ageing. Spinster homolog 1 (Spns1) is a transmembrane transporter, regulates autolysosomal biogenesis and plays a role in cellular senescence and survival. Using senescence‐associated β‐galactosidase staining, we found that carbofuran accelerates the cellular senescence in spns1 mutant zebrafish. The yolk opaqueness, a premature ageing phenotype in zebrafish embryos, was accelerated by carbofuran treatment. In the survival study, carbofuran shortened the life span of spns1 mutant zebrafish. Autophagy is the cellular lysosomal degradation, usually up‐regulated in the senescent cells. To know the impact of carbofuran exposure on autophagy progress, we established a double‐transgenic zebrafish line, harbouring EGFP‐tagged LC3‐II and mCherry‐tagged Lamp1 on spns1 mutant background, whereas we found, carbofuran exposure synergistically accelerates autolysosome formation with insufficient lysosome‐mediated degradation. Our data collectively suggest that carbofuran exposure synergistically accelerates the cellular senescence and affects biological ageing in spns1 defective animals.
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.16171