Measuring the subcellular compartmentalization of viral infections by protein complementation assay

The recent emergence and reemergence of viruses in the human population has highlighted the need to develop broader panels of therapeutic molecules. High-throughput screening assays opening access to untargeted steps of the viral replication cycle will provide powerful leverage to identify innovativ...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2021-01, Vol.118 (2), p.1-11
Main Authors: Fernandez, Juliette, Hassen-Khodja, Cédric, Georget, Virginie, Rose, Thierry, Jacob, Yves, Janin, Yves L., Nisole, Sébastien, Vidalain, Pierre-Olivier, Arhel, Nathalie J.
Format: Article
Language:English
Subjects:
Biological Sciences
Cell Line
Cell Nucleus - metabolism
Cytoplasm - metabolism
Green Fluorescent Proteins - metabolism
HeLa Cells
High-Throughput Screening Assays - methods
HIV Infections - metabolism
Humans
Life Sciences
Nuclear Envelope - metabolism
Nuclear Pore - metabolism
Ribonucleoproteins - metabolism
Virus Diseases - metabolism
Virus Replication - drug effects
Virus Replication - physiology
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Summary:The recent emergence and reemergence of viruses in the human population has highlighted the need to develop broader panels of therapeutic molecules. High-throughput screening assays opening access to untargeted steps of the viral replication cycle will provide powerful leverage to identify innovative antiviral molecules. We report here the development of an innovative protein complementation assay, termed αCentauri, to measure viral translocation between subcellular compartments. As a proof of concept, the Centauri fragment was either tethered to the nuclear pore complex or sequestered in the nucleus, while the complementary α fragment (6 amino acids) was attached to the integrase proteins of infectious HIV-1. The translocation of viral ribonucleoproteins from the cytoplasm to the nuclear envelope or to the nucleoplasm efficiently reconstituted superfolder green fluorescent protein or Nano-Luc αCentauri reporters. These fluorescence- or bioluminescence-based assays offer a robust readout of specific steps of viral infection in a multiwell format that is compatible for high-throughput screening and is validated by a short hairpin RNA-based prototype screen.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2010524118