Electrically tunable lens integrated with optical coherence tomography angiography for cerebral blood flow imaging in deep cortical layers in mice

We report the use of an electrically tunable lens (ETL) in a 1.3 μm spectral-domain optical coherence tomography (SD-OCT) system to overcome the depth of focus (DOF) limitation in conventional OCT systems for OCT angiography (OCTA) in a mouse cerebral cortex. The ETL provides fast and dynamic contro...

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Bibliographic Details
Published in:Optics letters 2019-10, Vol.44 (20), p.5037-5040
Main Authors: Li, Yuandong, Tang, Peijun, Song, Shaozhen, Rakymzhan, Adiya, Wang, Ruikang K
Format: Article
Language:English
Subjects:
Angiography
Animals
Blood flow
Cerebral cortex
Cerebral Cortex - blood supply
Cerebral Cortex - diagnostic imaging
Cerebrovascular Circulation
Depth of field
Dynamic control
Electricity
Flow velocity
Hemodynamics
Lenses
Medical imaging
Mice
Optical Coherence Tomography
Stitching
Tomography
Tomography, Optical Coherence - instrumentation
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Summary:We report the use of an electrically tunable lens (ETL) in a 1.3 μm spectral-domain optical coherence tomography (SD-OCT) system to overcome the depth of focus (DOF) limitation in conventional OCT systems for OCT angiography (OCTA) in a mouse cerebral cortex. The ETL provides fast and dynamic control of the axial focus of the probe beam along the entire range of the mouse cortex, upon which we performed cerebral blood flow imaging of all cortical layers by stitching the OCTA images automatically captured at six focal depths. Capillary vasculature and axial blood flow velocity were revealed in distinctive cortical layers and, for the first time, to the best of our knowledge, in white matter. The results have shown the system capability to conveniently investigate the hemodynamics in deep cortical layers in the mouse brain. More importantly, the compact integration of an ETL will benefit the future design of handheld or intra-cavity OCT probes for a wide range of applications in research and clinical fields.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.44.005037