Adoptive Transfer of Group 3-Like Innate Lymphoid Cells Restores Mouse Colon Resistance to Colonization of a Gamma Interferon-Susceptible Chlamydia muridarum Mutant

• Published in: Infection and immunity 2021-01, Vol.89 (2)
• Main Authors: He, Ying, Xu, Hong, Song, Chenchen, Koprivsek, John J, Arulanandam, Bernard, Yang, Huixiang, Tao, Lijian, Zhong, Guangming
• Format: Article
• Language: English
• Subjects: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00533-20

The obligate intracellular bacterium can colonize the mouse colon for a long period, but a gamma interferon (IFN-γ)-susceptible mutant clone fails to do so. Nevertheless, the mutant's colonization is rescued in mice deficient in interleukin-7 receptor (IL-7R) (lacking both lymphocytes and innate lymphoid cells [ILCs]) or IFN-γ but not in mice lacking recombination-activated gene 1 (Rag1 mice) (lacking adaptive immunity lymphocytes), indicating a critical role of ILC-derived IFN-γ in regulating chlamydial colonization. In the current study, we have used an adoptive transfer approach for further characterizing the responsible ILCs. First, intestinal ILCs isolated from Rag1 mice were able to rescue IL-7R-deficient mice to restrict the colonization of the IFN-γ-susceptible mutant. Second, the responsible ILCs were localized to the intestinal lamina propria since ILCs from the lamina propria but not the intraepithelial compartment conferred the restriction. Third, lamina propria ILCs enriched for RORγt expression but not those negative for RORγt rescued the IL-7R-deficient mice to restrict mutant colonization, indicating a critical role of group 3-like ILCs (ILC3s) since RORγt is a signature transcriptional factor of ILC3s. Fourth, a portion of the ILC3s expressed IFN-γ, thus defined as ex-ILC3s, and the transfer of the ex-ILC3s conferred colon resistance to mutant colonization in IFN-γ-deficient mice. Finally, genetically labeled RORγt-positive (RORγt ) ILCs were able to inhibit mutant colonization. Thus, we have demonstrated that ILC3s are sufficient for regulating chlamydial colonization, laying a foundation for further revealing the mechanisms by which an obligate intracellular bacterium activates colonic ILC3s.