Long-Term Impact of Suppressive Antibiotic Therapy on Intestinal Microbiota

The aim was to describe the safety of indefinite administration of antibiotics, the so-called suppressive antibiotic therapy (SAT) and to provide insight into their impact on gut microbiota. 17 patients with SAT were recruited, providing a fecal sample. Bacterial composition was determined by 16S rD...

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Bibliographic Details
Published in:Genes 2020-12, Vol.12 (1), p.41
Main Authors: Escudero-Sánchez, Rosa, Ponce-Alonso, Manuel, Barragán-Prada, Hugo, Morosini, María Isabel, Cantón, Rafael, Cobo, Javier, Del Campo, Rosa
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Bacteria
Deoxyribonucleic acid
Discriminant analysis
DNA
Feces
Infections
Intestinal microflora
Intestine
Microbiota
Multidrug resistance
Multidrug resistant organisms
Multivariate analysis
Patients
Prostheses
rRNA 16S
Selective media
Statistical analysis
Sulfonamides
Taxonomy
β-Lactam antibiotics
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Summary:The aim was to describe the safety of indefinite administration of antibiotics, the so-called suppressive antibiotic therapy (SAT) and to provide insight into their impact on gut microbiota. 17 patients with SAT were recruited, providing a fecal sample. Bacterial composition was determined by 16S rDNA massive sequencing, and their viability was explored by PCR-DGGE with and without propidium monoazide. Presence of antibiotic multirresistant bacteria was explored through the culture of feces in selective media. High intra-individual variability in the genera distribution regardless of the antibiotic or antibiotic administration ingestion period, with few statistically significant differences detected by Bray-Curtis distance-based principle component analysis, permutational multivariate analysis of variance and linear discriminant analysis effect size analysis. However, the microbiota composition of patients treated with both beta-lactams and sulfonamides clustered by a heat map. Curiously, the detection of antibiotic resistant bacteria was almost anecdotic and CTX-M-15-producing were detected in two subjects. Our work demonstrates the overall clinical safety of SAT and the low rate of the selection of multidrug-resistant bacteria triggered by this therapy. We also describe the composition of intestinal microbiota under the indefinite use of antibiotics for the first time.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes12010041