Mutations Affecting HVO_1357 or HVO_2248 Cause Hypermotility in Haloferax volcanii , Suggesting Roles in Motility Regulation

Motility regulation plays a key role in prokaryotic responses to environmental stimuli. Here, we used a motility screen and selection to isolate hypermotile mutants from a transposon insertion library. Whole genome sequencing revealed that hypermotile mutants were predominantly affected in two genes...

Full description

Saved in:
Bibliographic Details
Published in:Genes 2020-12, Vol.12 (1), p.58
Main Authors: Collins, Michiyah, Afolayan, Simisola, Igiraneza, Aime B, Schiller, Heather, Krespan, Elise, Beiting, Daniel P, Dyall-Smith, Mike, Pfeiffer, Friedhelm, Pohlschroder, Mechthild
Format: Article
Language:English
Subjects:
Bacteriorhodopsin
Biofilms
Biosynthesis
Chemotaxis
Deoxyribonucleic acid
DNA
Environmental effects
Gene regulation
Genomes
Haloferax volcanii
Histidine
Histidine kinase
Kinases
Motility
Mutants
Mutation
Proteins
Signal transduction
Stabbings
Transcription
Transposons
Whole genome sequencing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Motility regulation plays a key role in prokaryotic responses to environmental stimuli. Here, we used a motility screen and selection to isolate hypermotile mutants from a transposon insertion library. Whole genome sequencing revealed that hypermotile mutants were predominantly affected in two genes that encode HVO_1357 and HVO_2248. Alterations of these genes comprised not only transposon insertions but also secondary genome alterations. HVO_1357 contains a domain that was previously identified in the regulation of bacteriorhodopsin transcription, as well as other domains frequently found in two-component regulatory systems. The genes adjacent to encode a sensor box histidine kinase and a response regulator, key players of a two-component regulatory system. None of the homologues of HVO_2248 have been characterized, nor does it contain any of the assigned InterPro domains. However, in a significant number of species, the adjacent gene codes for a chemotaxis receptor/transducer. Our results provide a foundation for characterizing the root causes underlying hypermotility.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes12010058