Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2

DNA testing in cases of disputed paternity is a routine analysis carried out in genetic laboratories. The purpose of the test is to demonstrate similarities and differences in analyzed genetic markers between the alleged father, mother, and a child. The existence of differences in the examined loci...

Full description

Saved in:
Bibliographic Details
Published in:Genes 2021-01, Vol.12 (1), p.62
Main Authors: Doniec, Andrzej, Łuczak, Wojciech, Wróbel, Maria, Januła, Miłosz, Ossowski, Andrzej, Grzmil, Paweł, Kupiec, Tomasz
Format: Article
Language:English
Subjects:
Alleles
Chromosome 2
Chromosome aberrations
Chromosome deletion
Chromosomes
Data analysis
Deoxyribonucleic acid
DNA
Genetic analysis
Genetic markers
Genomes
Hypotheses
Insertion
Mutation
Paternity
Paternity testing
Sequence analysis
Single-nucleotide polymorphism
Software
Statistical analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DNA testing in cases of disputed paternity is a routine analysis carried out in genetic laboratories. The purpose of the test is to demonstrate similarities and differences in analyzed genetic markers between the alleged father, mother, and a child. The existence of differences in the examined loci between the child and the presumed father may indicate the exclusion of biological parenthood. However, another reason for such differences is genetic mutations, including chromosome aberrations and genome mutations. The presented results relate to genetic analyses carried out on three persons for the purposes of disputed paternity testing. A deviation from inheritance based on Mendel's Law was found in 7 out of 53 STR-type loci examined. All polymorphic loci that ruled out the paternity of the alleged father were located on chromosome 2. Additional analysis of 32 insertion-deletion markers (DIPplex, Qiagen) and sequencing of 94 polymorphic positions of the single nucleotide polymorphism (SNP) type (Illumina, ForenSeq) did not exclude the defendant's biological paternity. A sequence analysis of STR alleles and their flanking regions confirmed the hypothesis that the alleles on chromosome 2 of the child may originate only from the mother. The results of the tests did not allow exclusion of the paternity of the alleged father, but are an example of uniparental maternal disomy, which is briefly described in the literature.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes12010062