Preoperative Typing of Thyroid and Parathyroid Tumors with a Combined Molecular Classifier

In previous studies, we described a method for detecting and typing malignant tumors of the thyroid gland in fine-needle aspiration biopsy samples via analysis of a molecular marker panel (normalized mRNA level; normalized microRNA-146b, -221, and -375 levels; mitochondrial-to-nuclear DNA ratio; and...

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Bibliographic Details
Published in:Cancers 2021-01, Vol.13 (2), p.237
Main Authors: Titov, Sergei E, Kozorezova, Evgeniya S, Demenkov, Pavel S, Veryaskina, Yulia A, Kuznetsova, Irina V, Vorobyev, Sergey L, Chernikov, Roman A, Sleptsov, Ilya V, Timofeeva, Nataliya I, Ivanov, Mikhail K
Format: Article
Language:English
Subjects:
Algorithms
Biopsy
Deoxyribonucleic acid
DNA
Endocrine system
Gene expression
MicroRNAs
miRNA
Mitochondrial DNA
Mutation
Papillary thyroid carcinoma
Parathyroid
Thyroid
Thyroid cancer
Tumors
Typing
Ultrasonic imaging
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Summary:In previous studies, we described a method for detecting and typing malignant tumors of the thyroid gland in fine-needle aspiration biopsy samples via analysis of a molecular marker panel (normalized mRNA level; normalized microRNA-146b, -221, and -375 levels; mitochondrial-to-nuclear DNA ratio; and mutation) in cytological preparations by quantitative PCR. In the present study, we aimed to estimate the specificity of the typing of different thyroid tumors by the proposed method. Fine-needle aspiration cytological preparations from 278 patients were used. The histological diagnosis was known for each sample. The positive and negative predictive values of the method assessed in this study were, respectively, 100% and 98% for papillary thyroid carcinoma ( = 63), 100% and 100% for medullary thyroid carcinoma ( = 19), 43.5% and 98% for follicular carcinoma ( = 15), and 86% and 100% for Hürthle cell carcinoma ( = 6). Thus, we demonstrate that the diagnostic panel, including the analysis of microRNA expression, mRNA expression, the mutation, and the mitochondrial-to-nuclear DNA ratio, allows the highly accurate identification of papillary thyroid carcinoma, medullary thyroid carcinoma, and Hürthle cell carcinoma but not malignant follicular tumors (positive predictive value was below 50%).
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers13020237