Metagenome Analysis of a Hydrocarbon-Degrading Bacterial Consortium Reveals the Specific Roles of BTEX Biodegraders

Environmental contamination by petroleum hydrocarbons is of concern due to the carcinogenicity and neurotoxicity of these compounds. Successful bioremediation of organic contaminants requires bacterial populations with degradative capacity for these contaminants. Through successive enrichment of mic...

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Bibliographic Details
Published in:Genes 2021-01, Vol.12 (1), p.98
Main Author: Eze, Michael O
Format: Article
Language:English
Subjects:
Bacteria
Benzene
Biodegradation
Bioremediation
Carcinogenicity
Catechol
Contaminants
Crude oil
Dehydrogenases
Deoxyribonucleic acid
Diesel
DNA
Environmental restoration
Enzymes
Ethylbenzene
Experiments
Genetic testing
Genomics
Hydrocarbons
Inoculation
Laboratories
Metabolic pathways
Metabolism
Microorganisms
Neural coding
Neurotoxicity
Nucleotide sequence
Oil fields
Petroleum
Soil microorganisms
Soil pollution
Taxonomy
Thermal cycling
Toluene
Xylene
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Summary:Environmental contamination by petroleum hydrocarbons is of concern due to the carcinogenicity and neurotoxicity of these compounds. Successful bioremediation of organic contaminants requires bacterial populations with degradative capacity for these contaminants. Through successive enrichment of microorganisms from a petroleum-contaminated soil using diesel fuel as the sole carbon and energy source, we successfully isolated a bacterial consortium that can degrade diesel fuel hydrocarbons. Metagenome analysis revealed the specific roles of different microbial populations involved in the degradation of benzene, toluene, ethylbenzene and xylene (BTEX), and the metabolic pathways involved in these reactions. One hundred and five putative coding DNA sequences were identified as responsible for both the activation of BTEX and central metabolism (ring-cleavage) of catechol and alkylcatechols during BTEX degradation. The majority of the Coding DNA sequences (CDSs) were affiliated to , which was also the dominant bacterial genus in the consortium. The inoculation of diesel fuel contaminated soils with the consortium resulted in approximately 70% hydrocarbon biodegradation, indicating the potential of the consortium for environmental remediation of petroleum hydrocarbons.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes12010098