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miR-301b-3p Regulates Breast Cancer Cell Proliferation, Migration, and Invasion by Targeting NR3C2

Objectives. Breast cancer is the most common malignant tumor among females, and miRNAs have been reported to play an important regulatory role in breast cancer progression. This study aimed to explore the function and underlying molecular mechanism of miR-301b-3p in breast cancer. Methods. Different...

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Published in:Journal of oncology 2021, Vol.2021, p.8810517-9
Main Authors: Fan, Yaohua, Li, Yan, Zhu, Yuzhang, Dai, Guiping, Wu, Dongjuan, Gao, Zhenzhen, Zhang, Lei, Xu, Danying
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container_title Journal of oncology
container_volume 2021
creator Fan, Yaohua
Li, Yan
Zhu, Yuzhang
Dai, Guiping
Wu, Dongjuan
Gao, Zhenzhen
Zhang, Lei
Xu, Danying
description Objectives. Breast cancer is the most common malignant tumor among females, and miRNAs have been reported to play an important regulatory role in breast cancer progression. This study aimed to explore the function and underlying molecular mechanism of miR-301b-3p in breast cancer. Methods. Differential analysis and survival analysis were performed based on the data accessed from the TCGA-BRCA dataset for identification of the target miRNA. Bioinformatics analysis was conducted to predict the downstream target gene of the miRNA. Real-time quantitative PCR was carried out to detect the expression of miR-301b-3p and nuclear receptor subfamily 3 group C member 2 (NR3C2). Western blot was used to assess the protein expression of NR3C2. Cell counting kit-8 assay was performed to evaluate the proliferation of breast cancer cells. Transwell assay was conducted to determine the migratory and invasive abilities of breast cancer cells. Dual-luciferase reporter assay was employed to verify the targeting relationship between miR-301b-3p and NR3C2. Results. miR-301b-3p was elevated in breast cancer cell lines and promoted cell proliferation, migration, and invasion in terms of its biological function in breast cancer. NR3C2 was validated as a direct target of miR-301b-3p via bioinformatics analysis and dual-luciferase reporter assay, and NR3C2 was downregulated in breast cancer cell lines. The rescue experiment indicated that NR3C2 was involved in the mechanism by which miR-301b-3p regulated the malignant phenotype of breast cancer cells. Conclusion. The present study revealed for the first time that miR-301b-3p could foster breast cancer cell proliferation, migration, and invasion by targeting NR3C2, unveiling that miR-301b-3p is a novel carcinogen in breast cancer.
doi_str_mv 10.1155/2021/8810517
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Breast cancer is the most common malignant tumor among females, and miRNAs have been reported to play an important regulatory role in breast cancer progression. This study aimed to explore the function and underlying molecular mechanism of miR-301b-3p in breast cancer. Methods. Differential analysis and survival analysis were performed based on the data accessed from the TCGA-BRCA dataset for identification of the target miRNA. Bioinformatics analysis was conducted to predict the downstream target gene of the miRNA. Real-time quantitative PCR was carried out to detect the expression of miR-301b-3p and nuclear receptor subfamily 3 group C member 2 (NR3C2). Western blot was used to assess the protein expression of NR3C2. Cell counting kit-8 assay was performed to evaluate the proliferation of breast cancer cells. Transwell assay was conducted to determine the migratory and invasive abilities of breast cancer cells. Dual-luciferase reporter assay was employed to verify the targeting relationship between miR-301b-3p and NR3C2. Results. miR-301b-3p was elevated in breast cancer cell lines and promoted cell proliferation, migration, and invasion in terms of its biological function in breast cancer. NR3C2 was validated as a direct target of miR-301b-3p via bioinformatics analysis and dual-luciferase reporter assay, and NR3C2 was downregulated in breast cancer cell lines. The rescue experiment indicated that NR3C2 was involved in the mechanism by which miR-301b-3p regulated the malignant phenotype of breast cancer cells. Conclusion. The present study revealed for the first time that miR-301b-3p could foster breast cancer cell proliferation, migration, and invasion by targeting NR3C2, unveiling that miR-301b-3p is a novel carcinogen in breast cancer.</description><identifier>ISSN: 1687-8450</identifier><identifier>EISSN: 1687-8450</identifier><identifier>DOI: 10.1155/2021/8810517</identifier><identifier>PMID: 33542733</identifier><language>eng</language><publisher>Egypt: Hindawi</publisher><subject>Analysis ; Binding sites ; Bioinformatics ; Breast cancer ; Cancer ; Cell adhesion &amp; migration ; Cell cycle ; Cell growth ; Datasets ; Development and progression ; Experiments ; Medical equipment and supplies industry ; Medical prognosis ; Medical test kit industry ; Metastasis ; MicroRNA ; Oncology, Experimental ; Prognosis ; Proteins ; Software ; Survival analysis</subject><ispartof>Journal of oncology, 2021, Vol.2021, p.8810517-9</ispartof><rights>Copyright © 2021 Yaohua Fan et al.</rights><rights>COPYRIGHT 2021 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2021 Yaohua Fan et al. 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Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2021 Yaohua Fan et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570t-5beac0dd04778c38e7a0b13ce1eee64d4523d7446c53a88df6d502efbd76f5103</citedby><cites>FETCH-LOGICAL-c570t-5beac0dd04778c38e7a0b13ce1eee64d4523d7446c53a88df6d502efbd76f5103</cites><orcidid>0000-0001-6798-9595</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2484138495/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2484138495?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,25753,27923,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33542733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Izadpanah, Reza</contributor><contributor>Reza Izadpanah</contributor><creatorcontrib>Fan, Yaohua</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Zhu, Yuzhang</creatorcontrib><creatorcontrib>Dai, Guiping</creatorcontrib><creatorcontrib>Wu, Dongjuan</creatorcontrib><creatorcontrib>Gao, Zhenzhen</creatorcontrib><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Xu, Danying</creatorcontrib><title>miR-301b-3p Regulates Breast Cancer Cell Proliferation, Migration, and Invasion by Targeting NR3C2</title><title>Journal of oncology</title><addtitle>J Oncol</addtitle><description>Objectives. Breast cancer is the most common malignant tumor among females, and miRNAs have been reported to play an important regulatory role in breast cancer progression. This study aimed to explore the function and underlying molecular mechanism of miR-301b-3p in breast cancer. Methods. Differential analysis and survival analysis were performed based on the data accessed from the TCGA-BRCA dataset for identification of the target miRNA. Bioinformatics analysis was conducted to predict the downstream target gene of the miRNA. Real-time quantitative PCR was carried out to detect the expression of miR-301b-3p and nuclear receptor subfamily 3 group C member 2 (NR3C2). Western blot was used to assess the protein expression of NR3C2. Cell counting kit-8 assay was performed to evaluate the proliferation of breast cancer cells. Transwell assay was conducted to determine the migratory and invasive abilities of breast cancer cells. Dual-luciferase reporter assay was employed to verify the targeting relationship between miR-301b-3p and NR3C2. Results. miR-301b-3p was elevated in breast cancer cell lines and promoted cell proliferation, migration, and invasion in terms of its biological function in breast cancer. NR3C2 was validated as a direct target of miR-301b-3p via bioinformatics analysis and dual-luciferase reporter assay, and NR3C2 was downregulated in breast cancer cell lines. The rescue experiment indicated that NR3C2 was involved in the mechanism by which miR-301b-3p regulated the malignant phenotype of breast cancer cells. Conclusion. The present study revealed for the first time that miR-301b-3p could foster breast cancer cell proliferation, migration, and invasion by targeting NR3C2, unveiling that miR-301b-3p is a novel carcinogen in breast cancer.</description><subject>Analysis</subject><subject>Binding sites</subject><subject>Bioinformatics</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cell adhesion &amp; migration</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Datasets</subject><subject>Development and progression</subject><subject>Experiments</subject><subject>Medical equipment and supplies industry</subject><subject>Medical prognosis</subject><subject>Medical test kit industry</subject><subject>Metastasis</subject><subject>MicroRNA</subject><subject>Oncology, Experimental</subject><subject>Prognosis</subject><subject>Proteins</subject><subject>Software</subject><subject>Survival analysis</subject><issn>1687-8450</issn><issn>1687-8450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNp9kU1P3DAQhq2KqsC2t54rSxxLir_tvVSCiLZIQNEKzpZjT4JR1lnsLBX_vkG7UHrh5LHm0aOZeRH6TMk3SqU8YoTRI2MokVS_Q3tUGV0ZIcnOq3oX7ZdyR4gSZK4-oF3OpWCa8z3ULOOi4oQ2FV_hBXTr3o1Q8EkGV0Zcu-Qh4xr6Hl_loY8tZDfGIR3ii9g9ly4FfJYeXJl-uHnE1y53MMbU4csFr9lH9L51fYFP23eGbn6cXte_qvPfP8_q4_PKS03GSjbgPAmBCK2N5wa0Iw3lHigAKBGEZDxoIZSX3BkTWhUkYdA2QatWUsJn6PvGu1o3Swge0phdb1c5Ll1-tIOL9v9Oire2Gx6sNoJPp5oEB1tBHu7XUEZ7N6xzmma2TBhBuRFz-Y_qXA82pnaYZH4Zi7fHaq4040zqtynDJZdkEs7Q4YbyeSglQ_syLSX2KV37lK7dpjvhX15v-AI_xzkBXzfAbUzB_Ylv6_4C4CapUw</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Fan, Yaohua</creator><creator>Li, Yan</creator><creator>Zhu, Yuzhang</creator><creator>Dai, Guiping</creator><creator>Wu, Dongjuan</creator><creator>Gao, Zhenzhen</creator><creator>Zhang, Lei</creator><creator>Xu, Danying</creator><general>Hindawi</general><general>John Wiley &amp; 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migration</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Datasets</topic><topic>Development and progression</topic><topic>Experiments</topic><topic>Medical equipment and supplies industry</topic><topic>Medical prognosis</topic><topic>Medical test kit industry</topic><topic>Metastasis</topic><topic>MicroRNA</topic><topic>Oncology, Experimental</topic><topic>Prognosis</topic><topic>Proteins</topic><topic>Software</topic><topic>Survival analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, Yaohua</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Zhu, Yuzhang</creatorcontrib><creatorcontrib>Dai, Guiping</creatorcontrib><creatorcontrib>Wu, Dongjuan</creatorcontrib><creatorcontrib>Gao, Zhenzhen</creatorcontrib><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Xu, Danying</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing &amp; 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Breast cancer is the most common malignant tumor among females, and miRNAs have been reported to play an important regulatory role in breast cancer progression. This study aimed to explore the function and underlying molecular mechanism of miR-301b-3p in breast cancer. Methods. Differential analysis and survival analysis were performed based on the data accessed from the TCGA-BRCA dataset for identification of the target miRNA. Bioinformatics analysis was conducted to predict the downstream target gene of the miRNA. Real-time quantitative PCR was carried out to detect the expression of miR-301b-3p and nuclear receptor subfamily 3 group C member 2 (NR3C2). Western blot was used to assess the protein expression of NR3C2. Cell counting kit-8 assay was performed to evaluate the proliferation of breast cancer cells. Transwell assay was conducted to determine the migratory and invasive abilities of breast cancer cells. Dual-luciferase reporter assay was employed to verify the targeting relationship between miR-301b-3p and NR3C2. Results. miR-301b-3p was elevated in breast cancer cell lines and promoted cell proliferation, migration, and invasion in terms of its biological function in breast cancer. NR3C2 was validated as a direct target of miR-301b-3p via bioinformatics analysis and dual-luciferase reporter assay, and NR3C2 was downregulated in breast cancer cell lines. The rescue experiment indicated that NR3C2 was involved in the mechanism by which miR-301b-3p regulated the malignant phenotype of breast cancer cells. Conclusion. The present study revealed for the first time that miR-301b-3p could foster breast cancer cell proliferation, migration, and invasion by targeting NR3C2, unveiling that miR-301b-3p is a novel carcinogen in breast cancer.</abstract><cop>Egypt</cop><pub>Hindawi</pub><pmid>33542733</pmid><doi>10.1155/2021/8810517</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6798-9595</orcidid><oa>free_for_read</oa></addata></record>
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subjects Analysis
Binding sites
Bioinformatics
Breast cancer
Cancer
Cell adhesion & migration
Cell cycle
Cell growth
Datasets
Development and progression
Experiments
Medical equipment and supplies industry
Medical prognosis
Medical test kit industry
Metastasis
MicroRNA
Oncology, Experimental
Prognosis
Proteins
Software
Survival analysis
title miR-301b-3p Regulates Breast Cancer Cell Proliferation, Migration, and Invasion by Targeting NR3C2
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