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Fire blight QTL analysis in a multi-family apple population identifies a reduced-susceptibility allele in ‘Honeycrisp’
Breeding apple cultivars with resistance offers a potential solution to fire blight, a damaging bacterial disease caused by Erwinia amylovora . Most resistance alleles at quantitative trait loci (QTLs) were previously characterized in diverse Malus germplasm with poor fruit quality, which reduces br...
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Published in: | Horticulture research 2021-02, Vol.8 (1), Article 28 |
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description | Breeding apple cultivars with resistance offers a potential solution to fire blight, a damaging bacterial disease caused by
Erwinia amylovora
. Most resistance alleles at quantitative trait loci (QTLs) were previously characterized in diverse
Malus
germplasm with poor fruit quality, which reduces breeding utility. This study utilized a pedigree-based QTL analysis approach to elucidate the genetic basis of resistance/susceptibility to fire blight from multiple genetic sources in germplasm relevant to U.S. apple breeding programs. Twenty-seven important breeding parents (IBPs) were represented by 314 offspring from 32 full-sib families, with ‘Honeycrisp’ being the most highly represented IBP. Analyzing resistance/susceptibility data from a two-year replicated field inoculation study and previously curated genome-wide single nucleotide polymorphism data, QTLs were consistently mapped on chromosomes (Chrs.) 6, 7, and 15. These QTLs together explained ~28% of phenotypic variation. The Chr. 6 and Chr. 15 QTLs colocalized with previously reported QTLs, while the Chr. 7 QTL is possibly novel. ‘Honeycrisp’ inherited a rare reduced-susceptibility allele at the Chr. 6 QTL from its grandparent ‘Frostbite’. The highly resistant IBP ‘Enterprise’ had at least one putative reduced-susceptibility allele at all three QTLs. In general, lower susceptibility was observed for individuals with higher numbers of reduced-susceptibility alleles across QTLs. This study highlighted QTL mapping and allele characterization of resistance/susceptibility to fire blight in complex pedigree-connected apple breeding germplasm. Knowledge gained will enable more informed parental selection and development of trait-predictive DNA tests for pyramiding favorable alleles and selection of superior apple cultivars with resistance to fire blight. |
doi_str_mv | 10.1038/s41438-021-00466-6 |
format | article |
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Erwinia amylovora
. Most resistance alleles at quantitative trait loci (QTLs) were previously characterized in diverse
Malus
germplasm with poor fruit quality, which reduces breeding utility. This study utilized a pedigree-based QTL analysis approach to elucidate the genetic basis of resistance/susceptibility to fire blight from multiple genetic sources in germplasm relevant to U.S. apple breeding programs. Twenty-seven important breeding parents (IBPs) were represented by 314 offspring from 32 full-sib families, with ‘Honeycrisp’ being the most highly represented IBP. Analyzing resistance/susceptibility data from a two-year replicated field inoculation study and previously curated genome-wide single nucleotide polymorphism data, QTLs were consistently mapped on chromosomes (Chrs.) 6, 7, and 15. These QTLs together explained ~28% of phenotypic variation. The Chr. 6 and Chr. 15 QTLs colocalized with previously reported QTLs, while the Chr. 7 QTL is possibly novel. ‘Honeycrisp’ inherited a rare reduced-susceptibility allele at the Chr. 6 QTL from its grandparent ‘Frostbite’. The highly resistant IBP ‘Enterprise’ had at least one putative reduced-susceptibility allele at all three QTLs. In general, lower susceptibility was observed for individuals with higher numbers of reduced-susceptibility alleles across QTLs. This study highlighted QTL mapping and allele characterization of resistance/susceptibility to fire blight in complex pedigree-connected apple breeding germplasm. Knowledge gained will enable more informed parental selection and development of trait-predictive DNA tests for pyramiding favorable alleles and selection of superior apple cultivars with resistance to fire blight.</description><identifier>ISSN: 2662-6810</identifier><identifier>EISSN: 2052-7276</identifier><identifier>DOI: 10.1038/s41438-021-00466-6</identifier><identifier>PMID: 33518709</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/208/2491 ; 631/208/711 ; 631/208/8 ; Agriculture ; Alleles ; Apples ; Bacterial diseases ; Biomedical and Life Sciences ; Blight ; Chromosomes ; Cultivars ; Deoxyribonucleic acid ; Disease resistance ; DNA ; Ecology ; Erwinia amylovora ; Fire damage ; Frost resistance ; Frostbite ; Fruits ; Gene mapping ; Genetic analysis ; Genomes ; Germplasm ; Inoculation ; Life Sciences ; Malus ; Nucleotides ; Offspring ; Pedigree ; Phenotypic variations ; Plant breeding ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant Sciences ; Polymorphism ; Population genetics ; Quantitative trait loci ; Single-nucleotide polymorphism ; Susceptibility</subject><ispartof>Horticulture research, 2021-02, Vol.8 (1), Article 28</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-bddce44e442127d9dae55a62b5326eeb5f4b17236daab6edda3264352f186ff43</citedby><cites>FETCH-LOGICAL-c474t-bddce44e442127d9dae55a62b5326eeb5f4b17236daab6edda3264352f186ff43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847996/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847996/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Kostick, Sarah A.</creatorcontrib><creatorcontrib>Teh, Soon Li</creatorcontrib><creatorcontrib>Norelli, John L.</creatorcontrib><creatorcontrib>Vanderzande, Stijn</creatorcontrib><creatorcontrib>Peace, Cameron</creatorcontrib><creatorcontrib>Evans, Kate M.</creatorcontrib><title>Fire blight QTL analysis in a multi-family apple population identifies a reduced-susceptibility allele in ‘Honeycrisp’</title><title>Horticulture research</title><addtitle>Hortic Res</addtitle><description>Breeding apple cultivars with resistance offers a potential solution to fire blight, a damaging bacterial disease caused by
Erwinia amylovora
. Most resistance alleles at quantitative trait loci (QTLs) were previously characterized in diverse
Malus
germplasm with poor fruit quality, which reduces breeding utility. This study utilized a pedigree-based QTL analysis approach to elucidate the genetic basis of resistance/susceptibility to fire blight from multiple genetic sources in germplasm relevant to U.S. apple breeding programs. Twenty-seven important breeding parents (IBPs) were represented by 314 offspring from 32 full-sib families, with ‘Honeycrisp’ being the most highly represented IBP. Analyzing resistance/susceptibility data from a two-year replicated field inoculation study and previously curated genome-wide single nucleotide polymorphism data, QTLs were consistently mapped on chromosomes (Chrs.) 6, 7, and 15. These QTLs together explained ~28% of phenotypic variation. The Chr. 6 and Chr. 15 QTLs colocalized with previously reported QTLs, while the Chr. 7 QTL is possibly novel. ‘Honeycrisp’ inherited a rare reduced-susceptibility allele at the Chr. 6 QTL from its grandparent ‘Frostbite’. The highly resistant IBP ‘Enterprise’ had at least one putative reduced-susceptibility allele at all three QTLs. In general, lower susceptibility was observed for individuals with higher numbers of reduced-susceptibility alleles across QTLs. This study highlighted QTL mapping and allele characterization of resistance/susceptibility to fire blight in complex pedigree-connected apple breeding germplasm. 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Erwinia amylovora
. Most resistance alleles at quantitative trait loci (QTLs) were previously characterized in diverse
Malus
germplasm with poor fruit quality, which reduces breeding utility. This study utilized a pedigree-based QTL analysis approach to elucidate the genetic basis of resistance/susceptibility to fire blight from multiple genetic sources in germplasm relevant to U.S. apple breeding programs. Twenty-seven important breeding parents (IBPs) were represented by 314 offspring from 32 full-sib families, with ‘Honeycrisp’ being the most highly represented IBP. Analyzing resistance/susceptibility data from a two-year replicated field inoculation study and previously curated genome-wide single nucleotide polymorphism data, QTLs were consistently mapped on chromosomes (Chrs.) 6, 7, and 15. These QTLs together explained ~28% of phenotypic variation. The Chr. 6 and Chr. 15 QTLs colocalized with previously reported QTLs, while the Chr. 7 QTL is possibly novel. ‘Honeycrisp’ inherited a rare reduced-susceptibility allele at the Chr. 6 QTL from its grandparent ‘Frostbite’. The highly resistant IBP ‘Enterprise’ had at least one putative reduced-susceptibility allele at all three QTLs. In general, lower susceptibility was observed for individuals with higher numbers of reduced-susceptibility alleles across QTLs. This study highlighted QTL mapping and allele characterization of resistance/susceptibility to fire blight in complex pedigree-connected apple breeding germplasm. Knowledge gained will enable more informed parental selection and development of trait-predictive DNA tests for pyramiding favorable alleles and selection of superior apple cultivars with resistance to fire blight.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33518709</pmid><doi>10.1038/s41438-021-00466-6</doi><oa>free_for_read</oa></addata></record> |
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subjects | 631/208/2491 631/208/711 631/208/8 Agriculture Alleles Apples Bacterial diseases Biomedical and Life Sciences Blight Chromosomes Cultivars Deoxyribonucleic acid Disease resistance DNA Ecology Erwinia amylovora Fire damage Frost resistance Frostbite Fruits Gene mapping Genetic analysis Genomes Germplasm Inoculation Life Sciences Malus Nucleotides Offspring Pedigree Phenotypic variations Plant breeding Plant Breeding/Biotechnology Plant Genetics and Genomics Plant Sciences Polymorphism Population genetics Quantitative trait loci Single-nucleotide polymorphism Susceptibility |
title | Fire blight QTL analysis in a multi-family apple population identifies a reduced-susceptibility allele in ‘Honeycrisp’ |
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