Functional conservation of the grapevine candidate gene INNER NO OUTER for ovule development and seed formation

Seedlessness represents a highly appreciated trait in table grapes. Based on an interesting case of seedless fruit production described in the crop species Annona squamosa , we focused on the Vitis vinifera INNER NO OUTER (INO) gene as a candidate. This gene encodes a transcription factor belonging...

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Bibliographic Details
Published in:Horticulture research 2021-02, Vol.8 (1), Article 29
Main Authors: di Rienzo, Valentina, Imanifard, Zahra, Mascio, Isabella, Gasser, Charles S., Skinner, Debra J., Pierri, Ciro Leonardo, Marini, Martina, Fanelli, Valentina, Sabetta, Wilma, Montemurro, Cinzia, Bellin, Diana
Format: Article
Language:English
Subjects:
631/449/2679/2682
631/449/711
Agricultural production
Agriculture
Arabidopsis
Asymmetry
Biomedical and Life Sciences
Breeding
Complementation
Crop production
Ecology
Grapevines
Integument
Life Sciences
Mutants
Mutation
Organs
Ovules
Phylogeny
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Sciences
Seeds
Species
Vitis vinifera
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Summary:Seedlessness represents a highly appreciated trait in table grapes. Based on an interesting case of seedless fruit production described in the crop species Annona squamosa , we focused on the Vitis vinifera INNER NO OUTER (INO) gene as a candidate. This gene encodes a transcription factor belonging to the YABBY family involved in the determination of abaxial identity in several organs. In Arabidopsis thaliana , this gene was shown to be essential for the formation and asymmetric growth of the ovule outer integument and its mutation leads to a phenotypic defect of ovules and failure in seed formation. In this study, we identified in silico the V. vinifera orthologue and investigated its phylogenetic relationship to INO genes from other species and its expression in different organs in seeded and seedless varieties. Applying cross-species complementation, we have tested its functionality in the Arabidopsis ino -1 mutant. We show that the V. vinifera INO successfully rescues the ovule outer integument growth and seeds set and also partially complements the outer integument asymmetric growth in the Arabidopsis mutant, differently from orthologues from other species. These data demonstrate that VviINO retains similar activity and protein targets in grapevine as in Arabidopsis. Potential implications for grapevine breeding are discussed.
ISSN:2662-6810
2052-7276
DOI:10.1038/s41438-021-00467-5