Discovery of Amoebicidal Compounds by Combining Computational and Experimental Approaches

• Published in: Antimicrobial agents and chemotherapy 2021-01, Vol.65 (2)
• Main Authors: Sebastián-Pérez, Víctor, Sifaoui, Ines, Reyes-Batlle, María, Domínguez-De Barros, Angélica, López-Arencibia, Atteneri, Campillo, Nuria E, Piñero, José E, Lorenzo-Morales, Jacob, Gil, Carmen
• Format: Article
• Language: English
• Subjects: Experimental Therapeutics
• ISSN: 0066-4804; 1098-6596
• DOI: 10.1128/AAC.01749-20

Pathogenic and opportunistic free-living amoebae such as spp. can cause keratitis ( keratitis [AK]), which may ultimately lead to permanent visual impairment or blindness. can also cause rare but usually fatal granulomatous amoebic encephalitis (GAE). Current therapeutic options for AK require a lengthy treatment with nonspecific drugs that are often associated with adverse effects. Recent developments in the field led us to target cAMP pathways, specifically phosphodiesterase. Guided by computational tools, we targeted the phosphodiesterase RegA. Computational studies led to the construction and validation of a homology model followed by a virtual screening protocol guided by induced-fit docking and chemical scaffold analysis using our medicinal and biological chemistry (MBC) chemical library. Subsequently, 18 virtual screening hits were prioritized for further testing against , identifying amoebicidal hits containing piperidine and urea imidazole cores. Promising activities were confirmed in the resistant cyst form of the amoeba and in additional clinical strains, increasing their therapeutic potential. Mechanism-of-action studies revealed that these compounds produce apoptosis through reactive oxygen species (ROS)-mediated mitochondrial damage. These chemical families show promise for further optimization to produce effective antiacanthamoebal drugs.