Analysis of a Preliminary microRNA Expression Signature in a Human Telangiectatic Osteogenic Sarcoma Cancer Cell Line

Telangiectatic osteosarcoma (TOS) is an aggressive variant of osteosarcoma (OS) with distinctive radiographic, gross, microscopic features, and prognostic implications. Despite several studies on OS, we are still far from understanding the molecular mechanisms of TOS. In recent years, many studies h...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-01, Vol.22 (3), p.1163
Main Authors: Palmini, Gaia, Romagnoli, Cecilia, Donati, Simone, Zonefrati, Roberto, Galli, Gianna, Marini, Francesca, Iantomasi, Teresa, Aldinucci, Alessandra, Leoncini, Gigliola, Franchi, Alessandro, Beltrami, Giovanni, Campanacci, Domenico Andrea, Capanna, Rodolfo, Brandi, Maria Luisa
Format: Article
Language:English
Subjects:
Biology
Biomarkers
Biomedical materials
Biopsy
Bone cancer
Bone Neoplasms - genetics
Bone Neoplasms - metabolism
Bone Neoplasms - pathology
Bones
Cancer therapies
Cell Line, Tumor
Chemotherapy
Fluorescent Antibody Technique
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Medical prognosis
Metastases
Metastasis
MicroRNAs
MicroRNAs - genetics
Microscopy
miRNA
Molecular modelling
Osteosarcoma
Osteosarcoma - genetics
Osteosarcoma - metabolism
Osteosarcoma - pathology
Sarcoma
Stem cells
Surgery
Transcriptome
Tumorigenesis
Tumors
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Summary:Telangiectatic osteosarcoma (TOS) is an aggressive variant of osteosarcoma (OS) with distinctive radiographic, gross, microscopic features, and prognostic implications. Despite several studies on OS, we are still far from understanding the molecular mechanisms of TOS. In recent years, many studies have demonstrated not only that microRNAs (miRNAs) are involved in OS tumorigenesis, development, and metastasis, but also that the presence in high-grade types of OS of cancer stem cells (CSCs) plays an important role in tumor progression. Despite these findings, nothing has been described previously about the expression of miRNAs and the presence of CSCs in human TOS. Therefore, we have isolated/characterized a putative CSC cell line from human TOS (TOS-CSCs) and evaluated the expression levels of several miRNAs in TOS-CSCs using real-time quantitative assays. We show, for the first time, the existence of CSCs in human TOS, highlighting the in vitro establishment of this unique stabilized cell line and an identification of a preliminary expression of the miRNA profile, characteristic of TOS-CSCs. These findings represent an important step in the study of the biology of one of the most aggressive variants of OS and the role of miRNAs in TOS-CSC behavior.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22031163