Influence of germinated brown rice‐based flour modified by MAse on type 2 diabetic mice and HepG2 cell cytotoxic capacity

This study aimed to discover whether using maltogenic amylase (MAse) to modify starch in germinated brown rice flour may enhance slow digestion starch and release more bioactive compounds (BCs) content. To achieve this aim, the starch was modified with four levels of MAse (0 U, 133 U, 266 U and 399...

Full description

Saved in:
Bibliographic Details
Published in:Food science & nutrition 2021-02, Vol.9 (2), p.781-793
Main Authors: Nguyen, Ngoc Thi Le, Nguyen, Binh Duong Thanh, Dai, Trang Thi Xuan, Co, Son Hong, Do, Thao Thi, Tong Thi, Anh Ngoc, Oladapo, Ibitoye Joshua, Nguyen Cong, Ha
Format: Article
Language:English
Subjects:
Acids
Arteriosclerosis
Atherosclerosis
Bioactive compounds
Butyric acid
Carbohydrates
cytotoxic activity
Cytotoxicity
Diabetes
Diabetes mellitus
Enzymes
Ferulic acid
Flour
Food
Functional foods & nutraceuticals
germinated brown rice
Glucan 1,4-a-maltohydrolase
Laboratory animals
maltogenic amylase
modified starch
Original Research
Rice
Stability analysis
Starch
starch digestion
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aimed to discover whether using maltogenic amylase (MAse) to modify starch in germinated brown rice flour may enhance slow digestion starch and release more bioactive compounds (BCs) content. To achieve this aim, the starch was modified with four levels of MAse (0 U, 133 U, 266 U and 399 U MAse/g flour) for 1 hr at pH 5 and then spray‐dried to make modified flour. The biochemical impacts of the products were then accessed in normal and type 2 diabetic mice for 4 weeks. The result showed that when the starch was modified by MAse 266 U/g, a significant reduction of rapidly digested starch to 22.35% from 61.56%, an increase in slowly digested starch to 33.09% while resistant starch as 2.92% corresponding to the increase of γ‐amino butyric acid to 528.1 ± 44.1 mg/L and 120.6 ± 10.9 mg/L of ferulic acid. The extract from modified flour showed very strong cytotoxic activity against HepG2 cell (>80% inhibition). The result in vivo showed that the type‐2 diabetic mice fed with this modified product could better improve the stability of the glycemic index. Also, atherosclerotic plaque assessment further supports these findings. The results indicated that BCs released considerably couple with the changes in starch properties caused by MAse enhanced the effectiveness of this product to diabetes as well as positive effect on cytotoxic activity against HepG2 cell. The lowercase letters (a, b, c, d) in superscript indicated a statistically significant difference between treatments in each time by column (p 
ISSN:2048-7177
2048-7177
DOI:10.1002/fsn3.2043