Melatonin levels and microRNA (miRNA) relative expression profile in the follicular ambient microenvironment in patients undergoing in vitro fertilization process

Purpose Intrafollicular fluid (IFF) melatonin plays a decisive role in maintaining granulosa cells’ DNA integrity and protects them against apoptosis. It reduces oxidative stress and improves the oocyte quality with a higher fertilization rate. Method This prospective study investigated the antioxid...

Full description

Saved in:
Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2021-02, Vol.38 (2), p.443-459
Main Authors: Khan, Haroon Latif, Bhatti, Shahzad, Abbas, Sana, Kaloglu, Celal, Qurat-ul-Ain Zahra, Syeda, Khan, Yousaf Latif, Hassan, Zahira, Turhan, Nilgün Öztürk, Aydin, Hikmet Hakan
Format: Article
Language:English
Subjects:
Antioxidants
Apoptosis
Cellular Microenvironment - genetics
Deoxyribonucleic acid
DNA
Embryos
Female
Fertilization in Vitro - trends
Follicular fluid
Gene Expression Regulation, Developmental - genetics
Granulosa cells
Gynecology
Human Genetics
Humans
In vitro fertilization
Medicine
Medicine & Public Health
Melatonin
Melatonin - genetics
Microenvironments
MicroRNAs
MicroRNAs - classification
MicroRNAs - genetics
miRNA
Oocytes
Ovarian Follicle - growth & development
Ovarian Follicle - metabolism
Oxidative stress
Reproductive Medicine
Reproductive Physiology and Disease
Transcriptome - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose Intrafollicular fluid (IFF) melatonin plays a decisive role in maintaining granulosa cells’ DNA integrity and protects them against apoptosis. It reduces oxidative stress and improves the oocyte quality with a higher fertilization rate. Method This prospective study investigated the antioxidant property of IFF melatonin and its impact on IVF outcome parameters. We also explored the relative expression of five microRNAs (miR-663b, miR-320a, miR-766-3p, miR-132-3p, miR-16-5p) and levels of cell-free DNA (cfDNA) by real-time PCR in unexplained infertile patients. We collected 425 follicular fluid (FF) samples containing mature oocytes from 295 patients undergoing IVF. Results Patients were subgrouped based on IFF melatonin concentration (group A ≤ 30 pg/mL, group B > 70 to ≤ 110 pg/mL, group C > 111 to ≤ 385 pg/mL). Our results showed that patients with ≤ 30 pg/mL IFF melatonin levels have significantly higher oxidative stress markers, cfDNA levels, and lower relative expression of miR-663b, miR-320a, miR-766-3p, miR-132-3p, and miR-16-5p compared to other subgroups ( p  
ISSN:1058-0468
1573-7330
1573-7330
DOI:10.1007/s10815-020-02010-2