Rapid One-Pot Detection of SARS-CoV‑2 Based on a Lateral Flow Assay in Clinical Samples

Rapid tests for pathogen identification and spread assessment are critical for infectious disease control and prevention. The control of viral outbreaks requires a nucleic acid diagnostic test that is sensitive and simple and delivers fast and reliable results. Here, we report a one-pot direct rever...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-02, Vol.93 (7), p.3325-3330
Main Authors: Zhang, Chao, Zheng, Tingting, Wang, Hua, Chen, Wei, Huang, Xiaoye, Liang, Jianqi, Qiu, Liping, Han, Da, Tan, Weihong
Format: Article
Language:English
Subjects:
Assaying
Chemistry
COVID-19 - diagnosis
COVID-19 Testing
Diagnostic systems
Disease control
Gene amplification
Humans
Infectious diseases
Letter
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
Nucleic acids
Point-of-Care Testing
SARS-CoV-2
Sensitivity analysis
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Technicians
Time Factors
Transcription
Viral diseases
Workflow
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Summary:Rapid tests for pathogen identification and spread assessment are critical for infectious disease control and prevention. The control of viral outbreaks requires a nucleic acid diagnostic test that is sensitive and simple and delivers fast and reliable results. Here, we report a one-pot direct reverse transcript loop-mediated isothermal amplification (RT-LAMP) assay of SARS-CoV-2 based on a lateral flow assay in clinical samples. The entire contiguous sample-to-answer workflow takes less than 40 min from a clinical swab sample to a diagnostic result without professional instruments and technicians. The assay achieved an accuracy of 100% in 12 synthetic and 12 clinical samples compared to the data from PCR-based assays. We anticipate that our method will provide a universal platform for rapid and point-of-care detection of emerging infectious diseases.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c05059