Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples

We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019....

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2021-08, Vol.40 (8), p.1743-1748
Main Authors: Kaku, Norihito, Hashiguchi, Kohji, Akamatsu, Norihiko, Wakigawa, Fumiko, Matsuda, Junichi, Komaru, Kenzo, Nakao, Takumi, Harada, Yosuke, Hara, Atsuko, Uno, Naoki, Sakamoto, Kei, Morinaga, Yoshitomo, Kitazaki, Takeshi, Hasegawa, Hiroo, Miyazaki, Taiga, Fukuda, Masaaki, Izumikawa, Koichi, Mukae, Hiroshi, Yanagihara, Katsunori
Format: Article
Language:English
Subjects:
Assaying
Biomedical and Life Sciences
Biomedicine
Brief Report
Influenza
Influenza A
Internal Medicine
Medical Microbiology
Polymerase chain reaction
Reverse transcription
Sensitivity
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Summary:We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-021-04193-7