Bulk volume susceptibility difference between deoxyhemoglobin and oxyhemoglobin for HbA and HbS: A comparative study

• Published in: Magnetic resonance in medicine 2021-06, Vol.85 (6), p.3383-3393
• Main Authors: Eldeniz, Cihat, Binkley, Michael M., Fields, Melanie, Guilliams, Kristin, Ragan, Dustin K., Chen, Yasheng, Lee, Jin‐Moo, Ford, Andria L., An, Hongyu
• Format: Article
• Language: English
• Subjects: Affinity; Anemia; blood; Comparative studies; Cytology; Deoxygenation; Erythrocytes; Fetuses; Functional magnetic resonance imaging; Health risks; Hemoglobin; Hemoglobin A; Hemoglobin, Sickle; Hemoglobins; Humans; Ischemia; Magnetic fields; Magnetic permeability; magnetic susceptibility; Morphology; Oxygen; Oxygen content; Oxygenation; Oxyhemoglobin; Oxyhemoglobins; Partial pressure; Sickle cell anemia; Sickle cell disease; Statistical models
• ISSN: 0740-3194; 1522-2594
• DOI: 10.1002/mrm.28668

Purpose Sickle cell anemia is a blood disorder that alters the morphology and the oxygen affinity of the red blood cells. Cerebral oxygen extraction fraction measurements using quantitative BOLD contrast have been used for assessing inadequate oxygen delivery and the subsequent risk of ischemic stroke in sickle cell anemia. The BOLD signal in MRI studies relies on Δχdo, the bulk volume susceptibility difference between fully oxygenated and fully deoxygenated blood. Several studies have measured Δχdo for normal hemoglobin A (HbA). However, it is not known whether the value is different for sickle hemoglobin. In this study, Δχdo was measured for both HbA and sickle hemoglobin. Methods Six sickle cell anemia patients and 6 controls were recruited. Various blood oxygenation levels were achieved through in vivo manipulations to keep the blood close to its natural state. To account for the differences in oxygen affinity, Hill’s equations were used to translate partial pressure of oxygen to oxygen saturation for HbA, sickle hemoglobin, and fetal hemoglobin (HbF) separately. The pH and PCO2 corrections were performed. Temperature and magnetic field drift were controlled for. A multivariate generalized linear mixed model with random participant effect was used. Results Assuming that Δχdo is similar for HbA and HbF and that ΔχmetHb is 5/4 of Δχdo for HbA, it was found that the Δχdo values for HbA and sickle hemoglobin were not statistically significantly different from each other. Conclusion The same Δχdo value can be used for both types of hemoglobin in quantitative BOLD analysis.