The clinical dynamic changes of macrophage phenotype and function in different stages of human wound healing and hypertrophic scar formation

The pathogenesis of hypertrophic scar (HS) is still poorly understood. Macrophages, especially the polarisation of that to M1 or M2, play a pivotal role in control of the degree of scar formation. Profiling of macrophage phenotypes in human specimens during long‐term period of wound healing and HS f...

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Bibliographic Details
Published in:International wound journal 2019-04, Vol.16 (2), p.360-369
Main Authors: Chen, Lin, Wang, Jianzhang, Li, Shengxu, Yu, Zhou, Liu, Bei, Song, Baoqiang, Su, Yingjun
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Biomarkers
Child
Child, Preschool
China
Cicatrix, Hypertrophic - genetics
Cicatrix, Hypertrophic - physiopathology
cytokines
Cytokines - metabolism
Female
Humans
hypertrophic scar
Infant
macrophage phenotype
Macrophages - cytology
Macrophages - physiology
Male
Middle Aged
Original
Phenotype
wound healing
Wound Healing - physiology
Young Adult
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Summary:The pathogenesis of hypertrophic scar (HS) is still poorly understood. Macrophages, especially the polarisation of that to M1 or M2, play a pivotal role in control of the degree of scar formation. Profiling of macrophage phenotypes in human specimens during long‐term period of wound healing and HS formation may provide valuable clinical evidence for understanding the pathology of human scars. Human wound and HS specimens were collected, the macrophage phenotype was identified by immunofluorescence, and biomarkers and cytokines associated with M1 and M2 macrophages were detected by RT‐PCR. The correlation between the macrophage phenotype and HS characteristics was analysed by linear regression analyses. We found excessive and persistent infiltration by M1 macrophages around the blood vessels in the superficial layer of the dermis at early wound tissues, whereas M2 macrophages predominated in later wound tissues and the proliferative phase of HS and were scattered throughout the dermis. The density of M1 macrophages was positively correlated with mRNA expression levels of tumour necrosis factor‐alpha (TNF‐α) and IL‐6. The density of M2 macrophages was positively correlated with ARG1 and negatively correlated with the duration of HS. The sequential infiltration by M1 macrophage and M2 macrophages in human wound and HS tissues was confirmed.
ISSN:1742-4801
1742-481X
1742-481X
DOI:10.1111/iwj.13041