Biodecolourisation of Reactive Red 120 as a Sole Carbon Source by a Bacterial Consortium-Toxicity Assessment and Statistical Optimisation

The application of microorganisms in azo dye remediation has gained significant attention, leading to various published studies reporting different methods for obtaining the best dye decolouriser. This paper investigates and compares the role of methods and media used in obtaining a bacterial consor...

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Bibliographic Details
Published in:International journal of environmental research and public health 2021-03, Vol.18 (5), p.2424
Main Authors: Manogaran, Motharasan, Yasid, Nur Adeela, Othman, Ahmad Razi, Gunasekaran, Baskaran, Halmi, Mohd Izuan Effendi, Shukor, Mohd Yunus Abd
Format: Article
Language:English
Subjects:
Acclimation
Acclimatization
Aerobic conditions
Ammonium
Ammonium sulfate
Azo Compounds - toxicity
Azo dyes
Bacteria
Biodegradation, Environmental
Bioremediation
Breakdowns
Carbon
Carbon sources
Color removal
Coloring Agents
Consortia
Decoloring
Decolorization
Dyes
Germination
Glucose
Hydrocarbons
Manufacturing
Metabolites
Microorganisms
Photocatalysis
Pollution
Pseudomonas aeruginosa
Response surface methodology
Seed germination
Sulfates
Textile industry
Toxicity
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Summary:The application of microorganisms in azo dye remediation has gained significant attention, leading to various published studies reporting different methods for obtaining the best dye decolouriser. This paper investigates and compares the role of methods and media used in obtaining a bacterial consortium capable of decolourising azo dye as the sole carbon source, which is extremely rare to find. It was demonstrated that a prolonged acclimation under low substrate availability successfully isolated a novel consortium capable of utilising Reactive Red 120 dye as a sole carbon source in aerobic conditions. This consortium, known as JR3, consists of strain MM01, strain MM05 and strain MM06. Decolourised metabolites of consortium JR3 showed an improvement in mung bean's seed germination and shoot and root length. One-factor-at-time optimisation characterisation showed maximal of 82.9% decolourisation at 0.7 g/L ammonium sulphate, pH 8, 35 °C, and RR120 concentrations of 200 ppm. Decolourisation modelling utilising response surface methodology (RSM) successfully improved decolourisation even more. RSM resulted in maximal decolourisation of 92.79% using 0.645 g/L ammonium sulphate, pH 8.29, 34.5 °C and 200 ppm RR120.
ISSN:1660-4601
1661-7827
1660-4601
DOI:10.3390/ijerph18052424