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Hsp70 acts as a fine-switch that controls E3 ligase CHIP-mediated TAp63 and ΔNp63 ubiquitination and degradation

Abstract The major clinical problem in human cancer is metastasis. Metastases are the cause of 90% of human cancer deaths. TAp63 is a critical suppressor of tumorigenesis and metastasis. ΔNp63 acts as a dominant-negative inhibitor to block the function of p53 and TAp63. Although several ubiquitin E3...

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Published in:Nucleic acids research 2021-03, Vol.49 (5), p.2740-2758
Main Authors: Wu, H Helena, Wang, Benfan, Armstrong, Stephen R, Abuetabh, Yasser, Leng, Sarah, Roa, Wilson H Y, Atfi, Azeddine, Marchese, Adriano, Wilson, Beverly, Sergi, Consolato, Flores, Elsa R, Eisenstat, David D, Leng, Roger P
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cited_by cdi_FETCH-LOGICAL-c412t-2cd4f736785f266d23f3a591d93fa6e47da6ab7bfebf1bacc07f47f970ee10ff3
cites cdi_FETCH-LOGICAL-c412t-2cd4f736785f266d23f3a591d93fa6e47da6ab7bfebf1bacc07f47f970ee10ff3
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container_title Nucleic acids research
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creator Wu, H Helena
Wang, Benfan
Armstrong, Stephen R
Abuetabh, Yasser
Leng, Sarah
Roa, Wilson H Y
Atfi, Azeddine
Marchese, Adriano
Wilson, Beverly
Sergi, Consolato
Flores, Elsa R
Eisenstat, David D
Leng, Roger P
description Abstract The major clinical problem in human cancer is metastasis. Metastases are the cause of 90% of human cancer deaths. TAp63 is a critical suppressor of tumorigenesis and metastasis. ΔNp63 acts as a dominant-negative inhibitor to block the function of p53 and TAp63. Although several ubiquitin E3 ligases have been reported to regulate p63 stability, the mechanism of p63 regulation remains partially understood. Herein, we show that CHIP, an E3 ligase with a U-box domain, physically interacts with p63 and promotes p63 degradation. Notably, Hsp70 depletion by siRNA stabilizes TAp63 in H1299 cells and destabilizes ΔNp63 in SCC9 cells. Loss of Hsp70 results in a reduction in the TAp63-CHIP interaction in H1299 cells and an increase in the interaction between ΔNp63 and CHIP in SCC9 cells. Our results reveal that Hsp70 acts as a molecular switch to control CHIP-mediated ubiquitination and degradation of p63 isoforms. Furthermore, regulation of p63 by the Hsp70-CHIP axis contributes to the migration and invasion of tumor cells. Hence, our findings demonstrate that Hsp70 is a crucial regulator of CHIP-mediated ubiquitination and degradation of p63 isoforms and identify a new pathway for maintaining TAp63 or ΔNp63 stability in cancers.
doi_str_mv 10.1093/nar/gkab081
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Metastases are the cause of 90% of human cancer deaths. TAp63 is a critical suppressor of tumorigenesis and metastasis. ΔNp63 acts as a dominant-negative inhibitor to block the function of p53 and TAp63. Although several ubiquitin E3 ligases have been reported to regulate p63 stability, the mechanism of p63 regulation remains partially understood. Herein, we show that CHIP, an E3 ligase with a U-box domain, physically interacts with p63 and promotes p63 degradation. Notably, Hsp70 depletion by siRNA stabilizes TAp63 in H1299 cells and destabilizes ΔNp63 in SCC9 cells. Loss of Hsp70 results in a reduction in the TAp63-CHIP interaction in H1299 cells and an increase in the interaction between ΔNp63 and CHIP in SCC9 cells. Our results reveal that Hsp70 acts as a molecular switch to control CHIP-mediated ubiquitination and degradation of p63 isoforms. Furthermore, regulation of p63 by the Hsp70-CHIP axis contributes to the migration and invasion of tumor cells. 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Metastases are the cause of 90% of human cancer deaths. TAp63 is a critical suppressor of tumorigenesis and metastasis. ΔNp63 acts as a dominant-negative inhibitor to block the function of p53 and TAp63. Although several ubiquitin E3 ligases have been reported to regulate p63 stability, the mechanism of p63 regulation remains partially understood. Herein, we show that CHIP, an E3 ligase with a U-box domain, physically interacts with p63 and promotes p63 degradation. Notably, Hsp70 depletion by siRNA stabilizes TAp63 in H1299 cells and destabilizes ΔNp63 in SCC9 cells. Loss of Hsp70 results in a reduction in the TAp63-CHIP interaction in H1299 cells and an increase in the interaction between ΔNp63 and CHIP in SCC9 cells. Our results reveal that Hsp70 acts as a molecular switch to control CHIP-mediated ubiquitination and degradation of p63 isoforms. Furthermore, regulation of p63 by the Hsp70-CHIP axis contributes to the migration and invasion of tumor cells. 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source Oxford Journals Open Access; PubMed Central
subjects Animals
Apoptosis
Cell Line, Tumor
Cell Movement
Cells, Cultured
HSP70 Heat-Shock Proteins - metabolism
HSP70 Heat-Shock Proteins - physiology
Humans
Mice
Molecular Biology
Neoplasm Invasiveness
Neoplasms - metabolism
Neoplasms - mortality
Trans-Activators - metabolism
Transcription Factors - metabolism
Transcriptional Activation
Tumor Suppressor Proteins - metabolism
Ubiquitin-Protein Ligases - metabolism
Ubiquitination
title Hsp70 acts as a fine-switch that controls E3 ligase CHIP-mediated TAp63 and ΔNp63 ubiquitination and degradation
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