Solar photon-Fenton process eliminates free plasmid DNA harboring antimicrobial resistance genes from wastewater

• Published in: Journal of environmental management 2021-05, Vol.285, p.112204, Article 112204
• Main Authors: Vilela, Pâmela B., Martins, Alessandra S., Starling, Maria Clara V.M., de Souza, Felipe A.R., Pires, Giovana F.F., Aguilar, Ananda P., Pinto, Maria Eduarda A., Mendes, Tiago A.O., de Amorim, Camila C.
• Format: Article
• Language: English
• Subjects: Advanced oxidation processes; Anti-Bacterial Agents; Antimicrobial resistance; DNA; Drug Resistance, Bacterial - genetics; Escherichia coli - genetics; Hydrogen Peroxide; Neutral photo-Fenton; Oxidation-Reduction; Plasmids; Plasmids - genetics; Short Communication; Wastewater; Water Pollutants, Chemical
• ISSN: 0301-4797; 1095-8630; 1095-8630
• DOI: 10.1016/j.jenvman.2021.112204

This work aimed to assess the elimination and inactivation of resistance-conferring plasmids (RCPs) present in suspension in secondary wastewater by solar photo-Fenton as these are important vectors for the dissemination of antimicrobial resistance. Experiments were performed in synthetic secondary wastewater (SWW) and municipal wastewater treatment plant effluent (MWWTPE). Solar photo-Fenton (50 mg L−1 of H2O2 and 30 mg L−1 of Fe2+) was carried out for 60 min at neutral pH by applying the intermittent iron addition strategy. The removal of RCPs was assessed by Real-Time Polymerase Chain Reaction (qPCR). The transformation of competent non-resistant E. coli was used to evaluate the inactivation of target RCPs harboring antibiotic resistance genes (ARGs) to ampicillin (pSB1A2) or kanamycin (pSB1K3) after treatment and controls. Solar photo-Fenton completely removed RCPs initially present in both matrixes (SWW and MWWTPE), showing enhanced performance compared to the dark Fenton process. Both RCPs were inactivated after 30 min of solar photo-Fenton treatment, while 60 min were necessary to achieve the same effect for the dark Fenton reaction under similar conditions. These results indicate the potential of solar photo-Fenton to improve wastewater quality and reduce the spread of antimicrobial resistance in the environment by hampering the discharge of cell-free RCPs present in suspension in MWWTP onto environmental waters. [Display omitted] •Inactivation of plasmid via solar photo-Fenton assessed via E coli transformation.•Elimination of plasmid from wastewater via solar photo-Fenton assessed via qpCR.•Solar photo-Fenton removes plasmids carrying antibiotic resistance genes.•Solar photo-Fenton inactivates plasmids carrying antibiotic resistance genes.•Higher plasmid removal via solar photo-Fenton in simulated than real wastewater.