Specific, Surface-Driven, and High-Affinity Interactions of Fluorescent Hyaluronan with PEGylated Nanomaterials

Hybrid nanomaterials are a subject of extensive research in nanomedicine, and their clinical application is reasonably envisaged in the near future. However, the fate of nanomaterials in biological environments poses serious limitations to their application; therefore, schemes to monitor them and ga...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2020-02, Vol.12 (6), p.6806-6813
Main Authors: Palomba, Francesco, Rampazzo, Enrico, Zaccheroni, Nelsi, Malferrari, Marco, Rapino, Stefania, Greco, Valentina, Satriano, Cristina, Genovese, Damiano, Prodi, Luca
Format: Article
Language:English
Subjects:
Cell Membrane - chemistry
Cell Membrane - metabolism
Fluorescence
HeLa Cells
Humans
Hyaluronic Acid - chemistry
Hyaluronic Acid - metabolism
Nanoparticles - chemistry
Nanoparticles - metabolism
Nanostructures - chemistry
Polyethylene Glycols - chemistry
Rhodamines - chemistry
Rhodamines - metabolism
Silicon Dioxide - chemistry
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Summary:Hybrid nanomaterials are a subject of extensive research in nanomedicine, and their clinical application is reasonably envisaged in the near future. However, the fate of nanomaterials in biological environments poses serious limitations to their application; therefore, schemes to monitor them and gain control on their toxicity could be of great help for the development of the field. Here, we propose a probe for PEGylated nanosurfaces based on hyaluronic acid (HA) functionalized with rhodamine B (RB). We show that the high-affinity interaction of this fluorogenic hyaluronan (HA-RB) with nanoparticles exposing PEGylated surfaces results in their sensing, labeling for super-resolution imaging, and synergistic cellular internalization. HA-RB forms nanogels that interact with high affinitydown to the picomolar rangewith silica nanoparticles, selectively when their surface is covered by a soft and amphiphilic layer. This surface-driven interaction triggers the enhancement of the luminescence intensity of the dyes, otherwise self-quenched in HA-RB nanogels. The sensitive labeling of specific nanosurfaces also allowed us to obtain their super-resolution imaging via binding-activated localization microscopy (BALM). Finally, we show how this high-affinity interaction activates a synergistic cellular uptake of silica nanoparticles and HA-RB nanogels, followed by a differential fate of the two partner nanomaterials inside cells.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.9b17974