Triggering Neurotransmitters Secretion from Single Cells by X‑ray Nanobeam Irradiation

The employment of ionizing radiation is a powerful tool in cancer therapy, but beyond targeted effects, many studies have highlighted the relevance of its off-target consequences. An exhaustive understanding of the mechanisms underlying these effects is still missing, and no real-time data about sig...

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Bibliographic Details
Published in:Nano letters 2020-05, Vol.20 (5), p.3889-3894
Main Authors: Picollo, Federico, Tomagra, Giulia, Bonino, Valentina, Carabelli, Valentina, Mino, Lorenzo, Olivero, Paolo, Pasquarelli, Alberto, Truccato, Marco
Format: Article
Language:English
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Letter
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Summary:The employment of ionizing radiation is a powerful tool in cancer therapy, but beyond targeted effects, many studies have highlighted the relevance of its off-target consequences. An exhaustive understanding of the mechanisms underlying these effects is still missing, and no real-time data about signals released by cells during irradiation are presently available. We employed a synchrotron X-ray nanobeam to perform the first real-time simultaneous measurement of both X-ray irradiation and in vitro neurotransmitter release from individual adrenal phaeochromocytoma (PC12) cells plated over a diamond-based multielectrode array. We have demonstrated that, in specific conditions, X-rays can alter cell activity by promoting dopamine exocytosis, and such an effect is potentially very attractive for a more effective treatment of tumors.
ISSN:1530-6984
1530-6992
DOI:10.1021/acs.nanolett.0c01046