Fabrication and Impact of Fouling-Reducing Temperature-Responsive POEGMA Coatings with Embedded CaCO3 Nanoparticles on Different Cell Lines

In the present work, we have successfully prepared and characterized novel nanocomposite material exhibiting temperature-dependent surface wettability changes, based on grafted brush coatings of non-fouling poly(di(ethylene glycol)methyl ether methacrylate) (POEGMA) with the embedded CaCO3 nanoparti...

Full description

Saved in:
Bibliographic Details
Published in:Materials 2021-03, Vol.14 (6), p.1417
Main Authors: Lishchynskyi, Ostap, Stetsyshyn, Yurij, Raczkowska, Joanna, Awsiuk, Kamil, Orzechowska, Barbara, Abalymov, Anatolii, Skirtach, Andre G., Bernasik, Andrzej, Nastyshyn, Svyatoslav, Budkowski, Andrzej
Format: Article
Language:English
Subjects:
Adsorption
Biocompatibility
Biomedical materials
Biotechnology
Brushes
Calcium carbonate
Calcium chloride
Calcium phosphates
Cell adhesion
Cell adhesion & migration
Cell culture
Chemical precipitation
Coatings
Composite materials
Contact angle
Ethylene glycol
Fouling
Glass substrates
Grafting
Hydroxyapatite
Melanoma
Microscopy
Mineralization
Nanocomposites
Nanoparticles
Nitrogen
Osteoblasts
Polymerization
Polymers
Proteins
Quantum dots
Sodium carbonate
Spectrum analysis
Substrates
Temperature dependence
Tissue engineering
Wettability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present work, we have successfully prepared and characterized novel nanocomposite material exhibiting temperature-dependent surface wettability changes, based on grafted brush coatings of non-fouling poly(di(ethylene glycol)methyl ether methacrylate) (POEGMA) with the embedded CaCO3 nanoparticles. Grafted polymer brushes attached to the glass surface were prepared in a three-step process using atom transfer radical polymerization (ATRP). Subsequently, uniform CaCO3 nanoparticles (NPs) embedded in POEGMA-grafted brush coatings were synthesized using biomineralized precipitation from solutions of CaCl2 and Na2CO3. An impact of the low concentration of the embedded CaCO3 NPs on cell adhesion and growth depends strongly on the type of studied cell line: keratinocytes (HaCaT), melanoma (WM35) and osteoblastic (MC3T3-e1). Based on the temperature-responsive properties of grafted brush coatings and CaCO3 NPs acting as biologically active substrate, we hope that our research will lead to a new platform for tissue engineering with modified growth of the cells due to the release of biologically active substances from CaCO3 NPs and the ability to detach the cells in a controlled manner using temperature-induced changes of the brush.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma14061417