NMR visibility of deuterium‐labeled liver glycogen in vivo

Purpose Deuterium metabolic imaging (DMI) combined with [6,6’‐2H2]‐glucose has the potential to detect glycogen synthesis in the liver. However, the similar chemical shifts of [6,6’‐2H2]‐glucose and [6,6’‐2H2]‐glycogen in the 2H NMR spectrum make unambiguous detection and separation difficult in viv...

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Bibliographic Details
Published in:Magnetic resonance in medicine 2021-07, Vol.86 (1), p.62-68
Main Authors: De Feyter, Henk M., Thomas, Monique A., Behar, Kevin L., Graaf, Robin A.
Format: Article
Language:English
Subjects:
Deuterium
DMI
Drinking water
Glucose
Glycogen
Glycogens
In vivo methods and tests
Line spectra
Liver
NMR
Nuclear magnetic resonance
Spectral line width
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Summary:Purpose Deuterium metabolic imaging (DMI) combined with [6,6’‐2H2]‐glucose has the potential to detect glycogen synthesis in the liver. However, the similar chemical shifts of [6,6’‐2H2]‐glucose and [6,6’‐2H2]‐glycogen in the 2H NMR spectrum make unambiguous detection and separation difficult in vivo, in contrast to comparable approaches using 13C MRS. Here the NMR visibility of 2H‐labeled glycogen is investigated to better understand its potential contribution to the observed signal in liver following administration of [6,6’‐2H2]‐glucose. Methods Mice were provided drinking water containing 2H‐labeled glucose. High‐resolution NMR analyses was performed of isolated liver glycogen in solution, before and after the addition of the glucose‐releasing enzyme amyloglucosidase. Results 2H‐labeled glycogen was barely detectable in solution using 2H NMR because of the very short T2 (
ISSN:0740-3194
1522-2594
DOI:10.1002/mrm.28717