Downregulation of MIR100HG Induces Apoptosis in Human Megakaryoblastic Leukemia Cells

Long noncoding ribonucleic acids (lncRNAs) are ribonucleic acid (RNA) molecules longer than 200 nucleotides without protein-coding capacity. Several studies have shown that lncRNAs play a pivotal role in the initiation, maintenance, and progression of acute myeloid leukemia (AML), which could make t...

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Bibliographic Details
Published in:Indian journal of hematology & blood transfusion 2021-04, Vol.37 (2), p.232-239
Main Authors: Bagheri, Parisa, Sharifi, Mohammadreza, Ghadiri, Ava
Format: Article
Language:English
Subjects:
Apoptosis
Blood Transfusion Medicine
Hematology
Human Genetics
Leukemia
Medical prognosis
Medicine
Medicine & Public Health
Oncology
Original
Original Article
Ribonucleic acid
RNA
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Summary:Long noncoding ribonucleic acids (lncRNAs) are ribonucleic acid (RNA) molecules longer than 200 nucleotides without protein-coding capacity. Several studies have shown that lncRNAs play a pivotal role in the initiation, maintenance, and progression of acute myeloid leukemia (AML), which could make them a promising candidate in the diagnosis and treatment of leukemia. Acute Megakaryoblastic leukemia (AMKL) is a rare form of AML with a poor prognosis and low survival. It has been reported that lncRNA MIR100HG is involved several types of malignancies. In the present study, MIR100HG was downregulated in a human acute megakaryoblastic leukemia cell line (M-07e) using Antisense LNA GapmeRs. In order to assess the expression level of MIR100HG, cell viability, apoptosis, and necrosis (late apoptosis), quantitative reverse transcription polymerase chain reaction (qRT-PCR), Methyl-thiazol Tetrazolium assay, AnnexinV, and propidium iodide staining was performed at different time points after the transfection. In addition, the expression level of TGFβ was evaluated by qRT-PCR. Our results revealed that inhibition of MIR100HG might serve as a new method for inhibition of the proliferation of AMKL cells and therefore, could be a promising approach in medicine for targeted therapy in AMKL.
ISSN:0971-4502
0974-0449
0974-0449
0971-4502
DOI:10.1007/s12288-020-01324-6