Fuzheng Yiliu Formula Regulates Tumor Invasion and Metastasis through Inhibition of WAVE3 Expression

Objective. To explore the mechanism of action of Fuzheng Yiliu formula (FZYLF) in regulation of the invasion and metastasis of MDA-MB-231/Adr human breast cancer cells through WAVE3. Methods. The MDA-MB-231/Adr cells with high invasive ability were screened by Transwell, and the plasmid with high WA...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2021, Vol.2021, p.8898668-14
Main Authors: Chen, Wen-li, Bai, Huan-huan, Liu, Li-wei, Chen, Hong-yu, Shi, Qi, Chang, Li-sheng, Gou, Xiao-jun, Qian, Jun
Format: Article
Language:English
Subjects:
Antibodies
Antitumor activity
Breast cancer
Cancer therapies
Cell proliferation
Chemotherapy
Chinese medicine
Drugs
Enzymes
Flow cytometry
Gelatinase B
Gene expression
Invasiveness
Laboratory animals
Lymphatic system
Medical prognosis
Metastases
Metastasis
mRNA
NF-κB protein
Polymerase chain reaction
Proteins
Transfection
Western blotting
Xenografts
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Summary:Objective. To explore the mechanism of action of Fuzheng Yiliu formula (FZYLF) in regulation of the invasion and metastasis of MDA-MB-231/Adr human breast cancer cells through WAVE3. Methods. The MDA-MB-231/Adr cells with high invasive ability were screened by Transwell, and the plasmid with high WAVE3 expression was made for transfection. Plasmid transfection efficiency and protein expression level were verified by polymerase chain reaction (PCR) and western blotting (WB). The effect of FZYLF on cell proliferation and invasion was investigated before and after WAVE3 silencing by flow cytometry. A nude mouse model of tumor metastasis was established to study the antitumor activity of FZYLF. Results. The expression levels of mRNA and proteins of intracellular WAVE3 increased significantly after plasmid transfection, mRNA from 1.37± 0.41 to 9.88 ± 1.31 and protein from 1 ± 0.08 to 5.09 ± 0.03 (P 
ISSN:1741-427X
1741-4288
DOI:10.1155/2021/8898668