The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, resea...

Full description

Saved in:
Bibliographic Details
Published in:Polymers 2021-03, Vol.13 (7), p.1026
Main Authors: Chiodi, Elisa, Marn, Allison M, Geib, Matthew T, Ünlü, M Selim
Format: Article
Language:English
Subjects:
Antibodies
Binding sites
Biomarkers
Coating effects
DNA chips
Immobilization
Medical research
Methods
Morphology
Nanoparticles
Peptides
Polymer coatings
Popularity
Proteins
Review
Sensors
Substrates
Surface chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules' functionalities is critically analyzed.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym13071026