QuEChERS LC-MS/MS Screening Method for Mycotoxin Detection in Cereal Products and Spices

We developed and validated a screening method for mycotoxin analysis in cereal products and spices. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) was used for the analysis. Dispersive solid-phase extractions (d-SPEs) were used for the extraction of...

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Bibliographic Details
Published in:International journal of environmental research and public health 2021-04, Vol.18 (7), p.3774
Main Authors: Pantano, Licia, La Scala, Ladislao, Olibrio, Francesco, Galluzzo, Francesco Giuseppe, Bongiorno, Carmelo, Buscemi, Maria Drussilla, Macaluso, Andrea, Vella, Antonio
Format: Article
Language:English
Subjects:
Aflatoxins
Cereals
Chromatography, High Pressure Liquid
Chromatography, Liquid
Deoxynivalenol
Edible Grain - chemistry
Food contamination & poisoning
Food Contamination - analysis
Fungi
High performance liquid chromatography
Laboratories
Liquid chromatography
Liver cancer
Mass spectrometry
Mass spectroscopy
Methods
Mycotoxins
Mycotoxins - analysis
Ochratoxin A
Solid phases
Solvents
Spices
Tandem Mass Spectrometry
Toxicity
Zearalenone
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Summary:We developed and validated a screening method for mycotoxin analysis in cereal products and spices. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) was used for the analysis. Dispersive solid-phase extractions (d-SPEs) were used for the extraction of samples. Ochratoxin A (OTA), zearalenone (ZEA), aflatoxins (AFLA; AFB , AFB , AFG , AFG ), deoxynivalenol (DON), fumonisin (FUMO; FB , FB , FB ), T2, and HT2 were validated in maize. AFLA and DON were validated in black pepper. The method satisfies the requirements of Commission Regulation (EC) no. 401/2006 and (EC) no. 1881/2006. The screening target concentration (STC) was under maximum permitted levels (MLs) for all mycotoxins validated. The method's performance was assessed by two different proficiencies and tested with 100 real samples.
ISSN:1660-4601
1661-7827
1660-4601
DOI:10.3390/ijerph18073774