Alterations of macrophage and neutrophil content in skeletal muscle of aged versus young mice

Background Skeletal muscle inflammation and oxidative stress are associated with aging‐related loss of muscle mass and may be attributable to alterations in the number and types of leukocytes in skeletal muscle. Here, we tested the hypothesis that aging changes the number and composition of leukocyt...

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Bibliographic Details
Published in:Muscle & nerve 2021-04, Vol.63 (4), p.600-607
Main Authors: Kawanishi, Noriaki, Machida, Shuichi
Format: Article
Language:English
Subjects:
Aging
Aging - physiology
Animals
Basic Science
Basic Science s
Biomarkers - metabolism
Flow cytometry
Gastrocnemius muscle
Immune system
increasing age
Inflammation
Inflammation - immunology
Inflammation - metabolism
Inflammatory diseases
Leukocytes
Leukocytes (mononuclear)
Leukocytes (neutrophilic)
loss of muscle mass
macrophage
Macrophages
Macrophages - cytology
Macrophages - immunology
Male
Mice
Mice, Inbred C57BL
Muscle, Skeletal - physiology
Muscles
Muscular Diseases - immunology
Muscular Diseases - metabolism
Musculoskeletal diseases
Musculoskeletal system
Neutrophils
Neutrophils - cytology
Neutrophils - immunology
Nitrotyrosine
Oxidative stress
Oxidative Stress - physiology
Skeletal muscle
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Summary:Background Skeletal muscle inflammation and oxidative stress are associated with aging‐related loss of muscle mass and may be attributable to alterations in the number and types of leukocytes in skeletal muscle. Here, we tested the hypothesis that aging changes the number and composition of leukocyte subsets in skeletal muscle tissue. Methods Skeletal muscle was sampled from 4‐mo‐old (young) and 27‐mo‐old (old) C57BL/6J mice. Mononuclear cells of the gastrocnemius muscle were isolated, and flow cytometry was used to characterize the number and types of immune cells. Results The number of neutrophils and Ly‐6C+ inflammatory macrophages in the skeletal muscle was significantly higher in old mice than in young mice. Inflammation and oxidative stress (measured using the markers phosphorylated JNK and nitrotyrosine) were also higher in the skeletal muscle of old mice than in that of young mice. Conclusions Increasing age promotes skeletal muscle inflammation and oxidative stress, as well as infiltration of inflammatory macrophages and neutrophils.
ISSN:0148-639X
1097-4598
DOI:10.1002/mus.27158