Nitric oxide effectively curtails neck bending and mitigates senescence in isolated flowers of Calendula officinalis L

• Published in: Physiology and molecular biology of plants 2021-04, Vol.27 (4), p.835-845
• Main Authors: Lone, Mohammad Lateef, Haq, Aehsan ul, Farooq, Sumira, Altaf, Foziya, Tahir, Inayatullah
• Format: Article
• Language: English
• Subjects: Antioxidants; Bending; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Calendula officinalis; Cell Biology; Distilled water; Flowers; Life Sciences; Longevity; Neck; Nitric oxide; Phenols; Plant Physiology; Plant Sciences; Research Article; Senescence; Sodium nitroprusside
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-021-00969-5

In recent years, there has been a considerable and renewed upsurge in research to ascertain the physiological and biochemical role of Nitric oxide (NO) in plants. The present investigation is focused to study the role of NO on neck bending associated with senescence and postharvest performance in isolated flowers of Calendula officinalis . The flower buds harvested at one day before anthesis stage were supplied with sodium nitroprusside (SNP) as a source of NO at different concentrations viz., 50, 100, 150 and 200 µM. A distinct set of flowers held in distilled water designated the control. The investigation revealed that SNP delayed the senescence in flowers of C. officinalis significantly manifested by prolonged longevity. The maximum longevity of 12 days was recorded in flowers supplemented with 100 µM SNP. The flowers held in distilled water (control) displayed early senescence symptoms and lasted for 6 days only. Our research suggested that improved flower longevity by SNP was commensurate with delayed neck bending, inhibition of bacterial growth in the vase, increased solution uptake, high membrane stability, besides an up-regulated activities of antioxidant enzymes in the tissue samples. In addition, the treated flowers exhibited increased content of  sugar fractions, total phenols and soluble proteins in the petal tissues compared to control. Further, 100 µM SNP was observed as most effective treatment and increased the longevity of flowers by 6 days. The concentration above 150 µM provoked early senescence compared to control, whereas concentration lower than 100 µM was less efficacious in improving the postharvest life and longevity of cut  Calendula  flowers.