Genome-Wide Identification of Genes Involved in Acid Stress Resistance of Salmonella Derby

Resistance to and survival under acidic conditions are critical for to infect the host. As one of the most prevalent serotypes identified in pigs and humans, how . Derby overcomes acid stress remains unclear. Here, we de novo sequenced the genome of a representative Derby strain 14T from our Derby s...

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Bibliographic Details
Published in:Genes 2021-03, Vol.12 (4), p.476
Main Authors: Gu, Dan, Xue, Han, Yuan, Xiaohui, Yu, Jinyan, Xu, Xiaomeng, Huang, Yu, Li, Mingzhu, Zhai, Xianyue, Pan, Zhiming, Zhang, Yunzeng, Jiao, Xinan
Format: Article
Language:English
Subjects:
Acids
Bacteria
Colon cancer
Colorectal cancer
CRISPR
E coli
Epidemiology
Epithelial cells
Genes
Genomes
Glycerol
Hogs
Infections
Laboratories
Pathogens
Plasmids
Pork
Salmonella
Serotypes
Virulence
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Summary:Resistance to and survival under acidic conditions are critical for to infect the host. As one of the most prevalent serotypes identified in pigs and humans, how . Derby overcomes acid stress remains unclear. Here, we de novo sequenced the genome of a representative Derby strain 14T from our Derby strain stock and identified its acid resistance-associated genes using Tn-seq analysis. A total of 35 genes, including those belonging to two-component systems (TCS) ( ), the CRISPR-Cas system ( ), and other systems, were identified as essential for 14T to survive under acid stress. The results demonstrated that the growth curve and survival ability of Δ and Δ were decreased under acid stress, and the adhesion and invasion abilities to the mouse colon cancer epithelial cells (MC38) of Δ were also decreased compared with the wild type strain, suggesting that the TCS CpxAR plays an essential role in the acid resistance and virulence of . Derby. Also, CasC and CasE were found to be responsible for acid resistance in . Derby. Our results indicate that acid stress induces multiple genes' expression to mediate the acid resistance of . Derby and enhance its pathogenesis during an infection.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes12040476