Transcriptome Analysis Revealed Ameliorative Effects of Bacillus Based Probiotic on Immunity, Gut Barrier System, and Metabolism of Chicken under an Experimentally Induced Eimeria tenella Infection

In this study, we performed transcriptome analysis in the cecum tissues of negative control untreated non-challenged (NC), positive control untreated challenged (PC), and ( ) fed challenged chickens (BS + ET) in order to examine the underlying potential therapeutic mechanisms of based probiotic feed...

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Bibliographic Details
Published in:Genes 2021-04, Vol.12 (4), p.536
Main Authors: Memon, Fareed Uddin, Yang, Yunqiao, Leghari, Imdad Hussain, Lv, Feifei, Soliman, Ahmed M, Zhang, Weiyu, Si, Hongbin
Format: Article
Language:English
Subjects:
Bacillus
Cecum
Diarrhea
Disease
Eimeria tenella
Extracellular matrix
Gene expression
Genomes
Homeostasis
Infections
MAP kinase
Poultry
Probiotics
Protein kinase
Signal transduction
Software
Toll-like receptors
Transcriptomes
Vaccines
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Summary:In this study, we performed transcriptome analysis in the cecum tissues of negative control untreated non-challenged (NC), positive control untreated challenged (PC), and ( ) fed challenged chickens (BS + ET) in order to examine the underlying potential therapeutic mechanisms of based probiotic feeding under an experimental ( ) infection. Our results for clinical parameters showed that birds in probiotic diet decreased the bloody diarrhea scores, oocyst shedding, and lesion scores compared to positive control birds. RNA-sequencing (RNA-seq) analysis revealed that in total, 2509 up-regulated and 2465 down-regulated differentially expressed genes (DEGs) were detected in the PC group versus NC group comparison. In the comparison of BS + ET group versus PC group, a total of 784 up-regulated and 493 down-regulated DEGs were found. Among them, several DEGs encoding proteins involved in immunity, gut barrier integrity, homeostasis, and metabolism were up-regulated by the treatment of probiotic. Functional analysis of DEGs also revealed that some gene ontology (GO) terms related with immunity, metabolism and cellular development were significantly affected by the exposure of probiotic. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis showed that the DEGs in the cecum of -fed challenged group were mainly participated in the pathways related with immunity and gut barrier integrity, included mitogen-activated protein kinase (MAPK) signaling pathway, toll-like receptor (TLR) signaling pathway, extracellular matrix (ECM)-receptor interaction, tight junction, and so on. Taken together, these results suggest that based probiotic modulate the immunity, maintain gut homeostasis as well as barrier system and improve chicken metabolism during infection.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes12040536