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Role of Annexin A1 in Squamous Cell Lung Cancer Progression

Lung cancer remains the primary cause of cancer-related death worldwide, and its molecular mechanisms of tumor progression need further characterization to improve the clinical management of affected patients. The role of Annexin A1 (ANXA1) in tumorigenesis and cancer progression in general and espe...

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Published in:	Disease markers 2021, Vol.2021, p.5520832-10
Main Authors:	Elakad, Omar, Li, Yuchan, Gieser, Natascha, Yao, Sha, Küffer, Stefan, Hinterthaner, Marc, Danner, Bernhard C., von Hammerstein-Equord, Alexander, Ströbel, Philipp, Bohnenberger, Hanibal
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Language:	English
Subjects:	Adenocarcinoma Antibodies Biotechnology Breast cancer Cell growth Cell migration Cell survival CRISPR Expression vectors Impact analysis Laboratories Lung cancer Metastases Metastasis Molecular modelling Patients Penicillin Protein expression Proteins Small cell lung carcinoma Survival Tumorigenesis Tumors
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creator	Elakad, Omar Li, Yuchan Gieser, Natascha Yao, Sha Küffer, Stefan Hinterthaner, Marc Danner, Bernhard C. von Hammerstein-Equord, Alexander Ströbel, Philipp Bohnenberger, Hanibal
description	Lung cancer remains the primary cause of cancer-related death worldwide, and its molecular mechanisms of tumor progression need further characterization to improve the clinical management of affected patients. The role of Annexin A1 (ANXA1) in tumorigenesis and cancer progression in general and especially in lung cancer remains to be controversial and seems to be highly tissue specific and inconsistent among tumor initiation, progression, and metastasis. In the current study, we investigated ANXA1 expression in 81 squamous cell lung cancer (SQCLC), 86 pulmonary adenocarcinoma (AC), and 30 small cell lung cancer (SCLC) patient-derived tissue samples and its prognostic impact on patient’s survival. Mechanistically, we analyzed the impact of ANXA1 expression on proliferation and migration of SQCLC cell lines using CRISPR-Cas9 and mammalian overexpression vectors. Strong expression of ANXA1 was significantly correlated to longer overall survival only in SQCLC patients (P=0.019). Overexpression of ANXA1 promoted proliferation in SQCLC cell lines but suppressed their migration, while knockout of ANXA1 promoted cell migration and suppressed proliferation. In conclusion, ANXA1 expression might elongate patients’ survival by inhibiting tumor cell migration and subsequent metastasis.
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The role of Annexin A1 (ANXA1) in tumorigenesis and cancer progression in general and especially in lung cancer remains to be controversial and seems to be highly tissue specific and inconsistent among tumor initiation, progression, and metastasis. In the current study, we investigated ANXA1 expression in 81 squamous cell lung cancer (SQCLC), 86 pulmonary adenocarcinoma (AC), and 30 small cell lung cancer (SCLC) patient-derived tissue samples and its prognostic impact on patient’s survival. Mechanistically, we analyzed the impact of ANXA1 expression on proliferation and migration of SQCLC cell lines using CRISPR-Cas9 and mammalian overexpression vectors. Strong expression of ANXA1 was significantly correlated to longer overall survival only in SQCLC patients (P=0.019). Overexpression of ANXA1 promoted proliferation in SQCLC cell lines but suppressed their migration, while knockout of ANXA1 promoted cell migration and suppressed proliferation. 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subjects	Adenocarcinoma Antibodies Biotechnology Breast cancer Cell growth Cell migration Cell survival CRISPR Expression vectors Impact analysis Laboratories Lung cancer Metastases Metastasis Molecular modelling Patients Penicillin Protein expression Proteins Small cell lung carcinoma Survival Tumorigenesis Tumors
title	Role of Annexin A1 in Squamous Cell Lung Cancer Progression
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