Transcriptome Analysis Reveals the Genes Involved in Growth and Metabolism in Muscovy Ducks

Muscovy ducks are among the best meat ducks in the world. The objective of this study was to identify genes related to growth metabolism through transcriptome analysis of the ileal tissue of Muscovy ducks. Duck ileum samples with the highest (H group, n=5) and lowest (L group, n=5) body weight were...

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Bibliographic Details
Published in:BioMed research international 2021, Vol.2021 (1), p.6648435-6648435
Main Authors: Wang, Xingxin, Xiao, Yingping, Yang, Hua, Lu, Lizhi, Liu, Xiuting, Lyu, Wentao
Format: Article
Language:English
Subjects:
Amino acids
Aquatic birds
Biomedical research
Body weight
Ducks
Encyclopedias
Gene expression
Gene sequencing
Genes
Genetic aspects
Genetic regulation
Genomes
Glutathione
Growth
Ileum
Meat
Metabolism
Nutrients
Phenotypes
Physiological aspects
Poultry
Protein turnover
Small intestine
Software
Transcriptomes
Waterfowl
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Summary:Muscovy ducks are among the best meat ducks in the world. The objective of this study was to identify genes related to growth metabolism through transcriptome analysis of the ileal tissue of Muscovy ducks. Duck ileum samples with the highest (H group, n=5) and lowest (L group, n=5) body weight were selected from two hundred 70-day-old Muscovy ducks for transcriptome analysis by RNA sequencing. In the screening of differentially expressed genes (DEGs) between the H and L groups, a total of 602 DEGs with a fold change no less than 2 were identified, among which 285 were upregulated and 317 were downregulated. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that glutathione metabolism, pyrimidine metabolism, and protein digestion and absorption processes played a vital role in regulating growth and metabolism. The results showed that 7 genes related to growth and metabolism, namely, ANPEP, ENPEP, UPP1, SLC2A2, SLC6A19, NME4, and LOC106034733, were significantly expressed in group H, which was consistent with the phenotype results. The validation of these 7 genes using real-time quantitative PCR results indicated that the expression level of ENPEP was significantly different between the H and L groups (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2021/6648435