Corneal Optical Coherence Tomography Speckle in Crosslinked and Untreated Rabbit Eyes in Response to Elevated Intraocular Pressure

• Published in: Translational vision science & technology 2021-04, Vol.10 (5), p.2-2
• Main Authors: Danielewska, Monika E, Antonczyk, Agnieszka, Andrade De Jesus, Danilo, Rogala, Maja M, Blonska, Anna, Cwirko, Marek, Kielbowicz, Zdzislaw, Iskander, D Robert
• Format: Article
• Language: English
• Subjects: Special Issue
• ISSN: 2164-2591; 2164-2591
• DOI: 10.1167/tvst.10.5.2

To ascertain the influence of intraocular pressure (IOP) on corneal optical coherence tomography (OCT) speckle in untreated and ultraviolet A-riboflavin induced corneal collagen crosslinked rabbit eyes. Left corneas of eight rabbits were de-epithelialized and crosslinked by applying riboflavin and 30-minute ultraviolet A light exposure. After enucleation (6 months after treatment), each eyeball (treated and untreated) was mounted in a measurement setup, in which IOP was increased from 15 to 45 mm Hg in steps of 5 mm Hg. At each IOP value, single B-scans of the central cornea were acquired three times with the spectral-domain OCT Copernicus-HR. Then, three regions of interest, including the anterior, posterior, and entire corneal stroma, were automatically extracted. Five different probability distributions were used as a model for the corneal speckle and the one with the best goodness of fit was chosen for further analysis. The generalized gamma distribution achieved the best goodness of fit and its scale (a) and shape (v) parameters statistically significantly changed with increasing IOP in the three regions of analysis (two-way repeated measures analysis of variance, all P