Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells

We generated antisense constructs targeting two of the five Rubisco small subunit genes (OsRBCS2 and 4) which account for between 30–40 % of the RBCS transcript abundance in leaf blades. The constructs were driven by a maize phosphoenolpyruvate carboxylase (PEPC) promoter known to have enriched expr...

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Published in:Journal of plant physiology 2021-05, Vol.260, p.153395-153395, Article 153395
Main Authors: Maheshwari, Chirag, Coe, Robert A., Karki, Shanta, Covshoff, Sarah, Tapia, Ronald, Tyagi, Aruna, Hibberd, Julian M., Furbank, Robert T., Quick, William Paul, Lin, Hsiang-Chun
Format: Article
Language:English
Subjects:
Accumulation
Antisense
Assimilation
C4 rice
Carbon dioxide
Crop yield
Genes
Leaves
Mesophyll
Oryza sativa
Oxygenase
Phosphoenolpyruvate carboxylase
Photosynthesis
Proteins
RBCS
Ribulose-bisphosphate carboxylase
Rubisco
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Summary:We generated antisense constructs targeting two of the five Rubisco small subunit genes (OsRBCS2 and 4) which account for between 30–40 % of the RBCS transcript abundance in leaf blades. The constructs were driven by a maize phosphoenolpyruvate carboxylase (PEPC) promoter known to have enriched expression in mesophyll cells (MCs). In the resulting lines leaf, Rubisco protein content was reduced by between 30–50 % and CO2 assimilation rate was limited under photorespiratory and non-photorespiratory conditions. A relationship between Rubisco protein content and CO2 assimilation rate was found. This was associated with a significant reduction in dry biomass accumulation and grain yield of between 37–70%. In addition to serving as a resource for reducing Rubisco accumulation in a cell-preferential manner, these lines allow us to characterize gene function and isoform specific suppression on photosynthesis and growth. Our results suggest that the knockdown of multiple genes is required to completely reduce Rubisco accumulation in MCs.
ISSN:0176-1617
1618-1328
DOI:10.1016/j.jplph.2021.153395