Horsing Around: Escherichia coli ST1250 of Equine Origin Harboring Epidemic IncHI1/ST9 Plasmid with blaCTX-M-1 and an Operon for Short-Chain Fructooligosaccharide Metabolism

The relatedness of the equine-associated Escherichia coli strain ST1250 and its single- and double-locus variants (ST1250-SLV/DLV), obtained from horses in Europe, was studied by comparative genome analysis. A total of 54 isolates of E. coli ST1250 and ST1250-SLV/DLV from healthy and hospitalized ho...

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Published in:Antimicrobial agents and chemotherapy 2021-04, Vol.65 (5), p.e02556-20
Main Authors: Valcek, Adam, Sismova, Petra, Nesporova, Kristina, Overballe-Petersen, Søren, Bitar, Ibrahim, Jamborova, Ivana, Kant, Arie, Hrabak, Jaroslav, Wagenaar, Jaap A, Madec, Jean-Yves, Damborg, Peter, van Duijkeren, Engeline, Ewers, Christa, Hordijk, Joost, Hasman, Henrik, Brouwer, Michael S. M, Dolejska, Monika
Format: Article
Language:English
Subjects:
Bacteriology
Epidemiology and Surveillance
Life Sciences
Microbiology and Parasitology
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Summary:The relatedness of the equine-associated Escherichia coli strain ST1250 and its single- and double-locus variants (ST1250-SLV/DLV), obtained from horses in Europe, was studied by comparative genome analysis. A total of 54 isolates of E. coli ST1250 and ST1250-SLV/DLV from healthy and hospitalized horses across Europe (Czech Republic [n = 23], The Netherlands [n = 18], Germany [n = 9], Denmark [n = 3], and France [n = 1]) from 2008 to 2017 were subjected to whole-genome sequencing. An additional 25 draft genome assemblies of E. coli ST1250 and ST1250-SLV/DLV were obtained from the public databases. The isolates were compared for genomic features, virulence genes, clade structure, and plasmid content. The complete nucleotide sequences of eight IncHI1/ST9 plasmids and one IncHI1/ST2 plasmid were obtained using long-read sequencing by PacBio or MinION. In the collection of 79 isolates, only 10 were phylogenetically close (98% similarity) regardless of country of origin and differed only in the structure and integration site of the multidrug resistance (MDR) region. E. coli ST1250 and ST1250-SLV/DLV are phylogenetically diverse strains associated with horses. A strong linkage of E. coli ST1250 with the epidemic multidrug resistance plasmid lineage IncHI1/ST9 carrying blaCTX-M-1 and the fos operon was identified.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.02556-20