Loading…

Nanomechanics of G-quadruplexes within the promoter of the KIT oncogene

Abstract G-quadruplexes (G4s) are tetrahelical DNA structures stabilized by four guanines paired via Hoogsteen hydrogen bonds into quartets. While their presence within eukaryotic DNA is known to play a key role in regulatory processes, their functional mechanisms are still under investigation. In t...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 2021-05, Vol.49 (8), p.4564-4573
Main Authors: Buglione, Enrico, Salerno, Domenico, Marrano, Claudia Adriana, Cassina, Valeria, Vesco, Guglielmo, Nardo, Luca, Dacasto, Mauro, Rigo, Riccardo, Sissi, Claudia, Mantegazza, Francesco
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c412t-ba71222533024fabb4a16d56fe09a55580c9f17b1460c7e2514ffc4d46fbf99b3
cites cdi_FETCH-LOGICAL-c412t-ba71222533024fabb4a16d56fe09a55580c9f17b1460c7e2514ffc4d46fbf99b3
container_end_page 4573
container_issue 8
container_start_page 4564
container_title Nucleic acids research
container_volume 49
creator Buglione, Enrico
Salerno, Domenico
Marrano, Claudia Adriana
Cassina, Valeria
Vesco, Guglielmo
Nardo, Luca
Dacasto, Mauro
Rigo, Riccardo
Sissi, Claudia
Mantegazza, Francesco
description Abstract G-quadruplexes (G4s) are tetrahelical DNA structures stabilized by four guanines paired via Hoogsteen hydrogen bonds into quartets. While their presence within eukaryotic DNA is known to play a key role in regulatory processes, their functional mechanisms are still under investigation. In the present work, we analysed the nanomechanical properties of three G4s present within the promoter of the KIT proto-oncogene from a single-molecule point of view through the use of magnetic tweezers (MTs). The study of DNA extension fluctuations under negative supercoiling allowed us to identify a characteristic fingerprint of G4 folding. We further analysed the energetic contribution of G4 to the double-strand denaturation process in the presence of negative supercoiling, and we observed a reduction in the energy required for strands separation.
doi_str_mv 10.1093/nar/gkab079
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8096272</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/nar/gkab079</oup_id><sourcerecordid>2512740932</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-ba71222533024fabb4a16d56fe09a55580c9f17b1460c7e2514ffc4d46fbf99b3</originalsourceid><addsrcrecordid>eNp9kM9LwzAYhoMobk5P3qUnEaQuSZO2uQgydA6HXuY5pGmyVtukS1p__Pe2bIpePH18fA_v-_EAcIrgFYIsmhrhputXkcGE7YEximIcEhbjfTCGEaQhgiQdgSPvXyBEBFFyCEZRlBIGYzIG80dhbK1kIUwpfWB1MA83nchd11TqQ_ngvWyL0gRtoYLG2dq2yg3UsD8sVoE10q6VUcfgQIvKq5PdnIDnu9vV7D5cPs0Xs5tlKAnCbZiJBGGMaRRBTLTIMiJQnNNYK8gEpTSFkmmUZIjEUCYKU0S0liQnsc40Y1k0Adfb3KbLapVLZVonKt64shbuk1tR8r8XUxZ8bd94CnsnCe4DLnYBzm465Vtel16qqhJG2c7zvhInpPc6oJdbVDrrvVP6pwZBPqjnvXq-U9_TZ78_-2G_XffA-RawXfNv0hcElI5G</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2512740932</pqid></control><display><type>article</type><title>Nanomechanics of G-quadruplexes within the promoter of the KIT oncogene</title><source>Oxford Journals Open Access Collection</source><source>PubMed Central</source><creator>Buglione, Enrico ; Salerno, Domenico ; Marrano, Claudia Adriana ; Cassina, Valeria ; Vesco, Guglielmo ; Nardo, Luca ; Dacasto, Mauro ; Rigo, Riccardo ; Sissi, Claudia ; Mantegazza, Francesco</creator><creatorcontrib>Buglione, Enrico ; Salerno, Domenico ; Marrano, Claudia Adriana ; Cassina, Valeria ; Vesco, Guglielmo ; Nardo, Luca ; Dacasto, Mauro ; Rigo, Riccardo ; Sissi, Claudia ; Mantegazza, Francesco</creatorcontrib><description>Abstract G-quadruplexes (G4s) are tetrahelical DNA structures stabilized by four guanines paired via Hoogsteen hydrogen bonds into quartets. While their presence within eukaryotic DNA is known to play a key role in regulatory processes, their functional mechanisms are still under investigation. In the present work, we analysed the nanomechanical properties of three G4s present within the promoter of the KIT proto-oncogene from a single-molecule point of view through the use of magnetic tweezers (MTs). The study of DNA extension fluctuations under negative supercoiling allowed us to identify a characteristic fingerprint of G4 folding. We further analysed the energetic contribution of G4 to the double-strand denaturation process in the presence of negative supercoiling, and we observed a reduction in the energy required for strands separation.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/gkab079</identifier><identifier>PMID: 33849064</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Molecular Biology</subject><ispartof>Nucleic acids research, 2021-05, Vol.49 (8), p.4564-4573</ispartof><rights>The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. 2021</rights><rights>The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-ba71222533024fabb4a16d56fe09a55580c9f17b1460c7e2514ffc4d46fbf99b3</citedby><cites>FETCH-LOGICAL-c412t-ba71222533024fabb4a16d56fe09a55580c9f17b1460c7e2514ffc4d46fbf99b3</cites><orcidid>0000-0002-9713-1415 ; 0000-0003-4257-2165</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096272/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096272/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,1604,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33849064$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buglione, Enrico</creatorcontrib><creatorcontrib>Salerno, Domenico</creatorcontrib><creatorcontrib>Marrano, Claudia Adriana</creatorcontrib><creatorcontrib>Cassina, Valeria</creatorcontrib><creatorcontrib>Vesco, Guglielmo</creatorcontrib><creatorcontrib>Nardo, Luca</creatorcontrib><creatorcontrib>Dacasto, Mauro</creatorcontrib><creatorcontrib>Rigo, Riccardo</creatorcontrib><creatorcontrib>Sissi, Claudia</creatorcontrib><creatorcontrib>Mantegazza, Francesco</creatorcontrib><title>Nanomechanics of G-quadruplexes within the promoter of the KIT oncogene</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>Abstract G-quadruplexes (G4s) are tetrahelical DNA structures stabilized by four guanines paired via Hoogsteen hydrogen bonds into quartets. While their presence within eukaryotic DNA is known to play a key role in regulatory processes, their functional mechanisms are still under investigation. In the present work, we analysed the nanomechanical properties of three G4s present within the promoter of the KIT proto-oncogene from a single-molecule point of view through the use of magnetic tweezers (MTs). The study of DNA extension fluctuations under negative supercoiling allowed us to identify a characteristic fingerprint of G4 folding. We further analysed the energetic contribution of G4 to the double-strand denaturation process in the presence of negative supercoiling, and we observed a reduction in the energy required for strands separation.</description><subject>Molecular Biology</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>TOX</sourceid><recordid>eNp9kM9LwzAYhoMobk5P3qUnEaQuSZO2uQgydA6HXuY5pGmyVtukS1p__Pe2bIpePH18fA_v-_EAcIrgFYIsmhrhputXkcGE7YEximIcEhbjfTCGEaQhgiQdgSPvXyBEBFFyCEZRlBIGYzIG80dhbK1kIUwpfWB1MA83nchd11TqQ_ngvWyL0gRtoYLG2dq2yg3UsD8sVoE10q6VUcfgQIvKq5PdnIDnu9vV7D5cPs0Xs5tlKAnCbZiJBGGMaRRBTLTIMiJQnNNYK8gEpTSFkmmUZIjEUCYKU0S0liQnsc40Y1k0Adfb3KbLapVLZVonKt64shbuk1tR8r8XUxZ8bd94CnsnCe4DLnYBzm465Vtel16qqhJG2c7zvhInpPc6oJdbVDrrvVP6pwZBPqjnvXq-U9_TZ78_-2G_XffA-RawXfNv0hcElI5G</recordid><startdate>20210507</startdate><enddate>20210507</enddate><creator>Buglione, Enrico</creator><creator>Salerno, Domenico</creator><creator>Marrano, Claudia Adriana</creator><creator>Cassina, Valeria</creator><creator>Vesco, Guglielmo</creator><creator>Nardo, Luca</creator><creator>Dacasto, Mauro</creator><creator>Rigo, Riccardo</creator><creator>Sissi, Claudia</creator><creator>Mantegazza, Francesco</creator><general>Oxford University Press</general><scope>TOX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9713-1415</orcidid><orcidid>https://orcid.org/0000-0003-4257-2165</orcidid></search><sort><creationdate>20210507</creationdate><title>Nanomechanics of G-quadruplexes within the promoter of the KIT oncogene</title><author>Buglione, Enrico ; Salerno, Domenico ; Marrano, Claudia Adriana ; Cassina, Valeria ; Vesco, Guglielmo ; Nardo, Luca ; Dacasto, Mauro ; Rigo, Riccardo ; Sissi, Claudia ; Mantegazza, Francesco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-ba71222533024fabb4a16d56fe09a55580c9f17b1460c7e2514ffc4d46fbf99b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Molecular Biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buglione, Enrico</creatorcontrib><creatorcontrib>Salerno, Domenico</creatorcontrib><creatorcontrib>Marrano, Claudia Adriana</creatorcontrib><creatorcontrib>Cassina, Valeria</creatorcontrib><creatorcontrib>Vesco, Guglielmo</creatorcontrib><creatorcontrib>Nardo, Luca</creatorcontrib><creatorcontrib>Dacasto, Mauro</creatorcontrib><creatorcontrib>Rigo, Riccardo</creatorcontrib><creatorcontrib>Sissi, Claudia</creatorcontrib><creatorcontrib>Mantegazza, Francesco</creatorcontrib><collection>Oxford Journals Open Access Collection</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buglione, Enrico</au><au>Salerno, Domenico</au><au>Marrano, Claudia Adriana</au><au>Cassina, Valeria</au><au>Vesco, Guglielmo</au><au>Nardo, Luca</au><au>Dacasto, Mauro</au><au>Rigo, Riccardo</au><au>Sissi, Claudia</au><au>Mantegazza, Francesco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanomechanics of G-quadruplexes within the promoter of the KIT oncogene</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>2021-05-07</date><risdate>2021</risdate><volume>49</volume><issue>8</issue><spage>4564</spage><epage>4573</epage><pages>4564-4573</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>Abstract G-quadruplexes (G4s) are tetrahelical DNA structures stabilized by four guanines paired via Hoogsteen hydrogen bonds into quartets. While their presence within eukaryotic DNA is known to play a key role in regulatory processes, their functional mechanisms are still under investigation. In the present work, we analysed the nanomechanical properties of three G4s present within the promoter of the KIT proto-oncogene from a single-molecule point of view through the use of magnetic tweezers (MTs). The study of DNA extension fluctuations under negative supercoiling allowed us to identify a characteristic fingerprint of G4 folding. We further analysed the energetic contribution of G4 to the double-strand denaturation process in the presence of negative supercoiling, and we observed a reduction in the energy required for strands separation.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>33849064</pmid><doi>10.1093/nar/gkab079</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-9713-1415</orcidid><orcidid>https://orcid.org/0000-0003-4257-2165</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0305-1048
ispartof Nucleic acids research, 2021-05, Vol.49 (8), p.4564-4573
issn 0305-1048
1362-4962
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8096272
source Oxford Journals Open Access Collection; PubMed Central
subjects Molecular Biology
title Nanomechanics of G-quadruplexes within the promoter of the KIT oncogene
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T09%3A51%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nanomechanics%20of%20G-quadruplexes%20within%20the%20promoter%20of%20the%20KIT%20oncogene&rft.jtitle=Nucleic%20acids%20research&rft.au=Buglione,%20Enrico&rft.date=2021-05-07&rft.volume=49&rft.issue=8&rft.spage=4564&rft.epage=4573&rft.pages=4564-4573&rft.issn=0305-1048&rft.eissn=1362-4962&rft_id=info:doi/10.1093/nar/gkab079&rft_dat=%3Cproquest_pubme%3E2512740932%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c412t-ba71222533024fabb4a16d56fe09a55580c9f17b1460c7e2514ffc4d46fbf99b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2512740932&rft_id=info:pmid/33849064&rft_oup_id=10.1093/nar/gkab079&rfr_iscdi=true