Promise and challenges of dystonia brain banking: establishing a human tissue repository for studies of X-Linked Dystonia-Parkinsonism

• Published in: Journal of Neural Transmission 2021-04, Vol.128 (4), p.575-587
• Main Authors: Fernandez-Cerado, Cara, Legarda, G. Paul, Velasco-Andrada, M. Salvie, Aguil, Abegail, Ganza-Bautista, Niecy G., Lagarde, J. Benedict B., Soria, Jasmin, Jamora, Roland Dominic G., Acuña, Patrick J., Vanderburg, Charles, Sapp, Ellen, DiFiglia, Marian, Murcar, Micaela G., Campion, Lindsey, Ozelius, Laurie J., Alessi, Amy K., Singh-Bains, Malvindar K., Waldvogel, Henry J., Faull, Richard L. M., Macalintal-Canlas, Regina, Muñoz, Edwin L., Penney, Ellen B., Ang, Mark A., Diesta, Cid Czarina E., Bragg, D. Cristopher, Acuña-Sunshine, Geraldine
• Format: Article
• Language: English
• Subjects: Medicine; Medicine & Public Health; Neurology; Neurology and Preclinical Neurological Studies - Original; Neurology and Preclinical Neurological Studies - Original Article; Neurosciences; Psychiatry
• ISSN: 0300-9564; 1435-1463
• DOI: 10.1007/s00702-020-02286-9

X-Linked Dystonia-Parkinsonism (XDP) is a neurodegenerative disease affecting individuals with ancestry to the island of Panay in the Philippines. In recent years there has been considerable progress at elucidating the genetic basis of XDP and candidate disease mechanisms in patient-derived cellular models, but the neural substrates that give rise to XDP in vivo are still poorly understood. Previous studies of limited XDP postmortem brain samples have reported a selective dropout of medium spiny neurons within the striatum, although neuroimaging of XDP patients has detected additional abnormalities in multiple brain regions beyond the basal ganglia. Given the need to fully define the CNS structures that are affected in this disease, we created a brain bank in Panay to serve as a tissue resource for detailed studies of XDP-related neuropathology. Here we describe this platform, from donor recruitment and consent to tissue collection, processing, and storage, that was assembled within a predominantly rural region of the Philippines with limited access to medical and laboratory facilities. Thirty-six brains from XDP individuals have been collected over an initial 4 years period. Tissue quality was assessed based on histologic staining of cortex, RNA integrity scores, detection of neuronal transcripts in situ by fluorescent hybridization chain reaction, and western blotting of neuronal and glial proteins. The results indicate that this pipeline preserves tissue integrity to an extent compatible with a range of morphologic, molecular, and biochemical analyses. Thus the algorithms that we developed for working in rural communities may serve as a guide for establishing similar brain banks for other rare diseases in indigenous populations.