Bacterial diversity changes in agricultural soils influenced by poultry litter fertilization

• Published in: Brazilian journal of microbiology 2021-06, Vol.52 (2), p.675-686
• Main Authors: Parente, Cláudio E. T., Brito, Elcia M. S., Caretta, César A., Cervantes-Rodríguez, Erick A., Fábila-Canto, Andrea P., Vollú, Renata E., Seldin, Lucy, Malm, Olaf
• Format: Article
• Language: English
• Subjects: Agricultural land; Agricultural wastes; Agriculture; Animals; Antibiotic resistance; Antibiotics; Bacteria; Bacteria - classification; Bacteria - growth & development; Bacteria - isolation & purification; Bacteria - metabolism; Biodiversity; Biomedical and Life Sciences; Contamination; Denitrification; Environmental Microbiology - Research Paper; Farm buildings; Feces - chemistry; Fertilization; Fertilizers - analysis; Food Microbiology; Forest soils; Genera; Greenhouse gases; Life Sciences; Litter; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microbiomes; Mycology; Next-generation sequencing; Nitrous oxide; Phylogeny; Poultry; Soil - chemistry; Soil contamination; Soil Microbiology; Soil microorganisms; Soils
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-021-00437-y

Poultry litter is widely applied as agricultural fertilizer and can affect the soil microbiome through nutrient overload and antibiotic contamination. In this study, we assessed changes in soil bacterial diversity using high-throughput sequencing approaches. Four samples in triplicate were studied: soils with short- and long-term fertilization by poultry litter (S1 = 10 months and S2 = 30 years, respectively), a soil inside a poultry shed (S3), and a forest soil used as control (S0). Samples S0, S1, and S2 revealed a relatively high richness, with confirmed operational taxonomic units (OTUs) in the three replicates of each sample ranging from 1243 to 1279, while richness in S3 was about three times lower (466). The most abundant phyla were Proteobacteria, Bacteroidetes, and Actinobacteria. Acidobacteria, Planctomycetes, and Verrucomicrobia were also abundant but highly diminished in S3, while Firmicutes was less abundant in S0. Changes in bacterial communities were very evident at the genera level. The genera Gaiella , Rhodoplanes, Solirubacter , and Sphingomonas were predominant in S0 but strongly decreased in the other soils. Pedobacter and Devosia were the most abundant in S1 and were diminished in S2, while Herbiconiux , Brevundimonas , Proteiniphilum , and Petrimonas were abundant in S2. The most abundant genera in S3 were Deinococcus , Truepera , Rhodanobacter , and Castellaniella . A predictive analysis of the metabolic functions with Tax4Fun2 software suggested the potential presence of enzymes associated with antibiotic resistance as well as with denitrification pathways, indicating that the S3 soil is a potential source of nitrous oxide, a powerful greenhouse gas.