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Maternal oxycodone treatment causes pathophysiological changes in the mouse placenta

Pregnant women are increasingly being prescribed and abusing opioid drugs. As the primary communication organ between mother and conceptus, the placenta may be vulnerable to opioid effects but also holds the key to better understanding how these drugs affect long-term offspring health. We hypothesiz...

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Published in:Placenta (Eastbourne) 2020-10, Vol.100, p.96-110
Main Authors: Green, Madison T., Martin, Rachel E., Kinkade, Jessica A., Schmidt, Robert R., Bivens, Nathan J., Tuteja, Geetu, Mao, Jiude, Rosenfeld, Cheryl S.
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container_title Placenta (Eastbourne)
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creator Green, Madison T.
Martin, Rachel E.
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Schmidt, Robert R.
Bivens, Nathan J.
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Mao, Jiude
Rosenfeld, Cheryl S.
description Pregnant women are increasingly being prescribed and abusing opioid drugs. As the primary communication organ between mother and conceptus, the placenta may be vulnerable to opioid effects but also holds the key to better understanding how these drugs affect long-term offspring health. We hypothesized that maternal treatment with oxycodone (OXY), the primary opioid at the center of the current crisis, deleteriously affects placental structure and gene expression patterns. Female mice were treated daily with 5 mg OXY/kg or saline solution (Control, CTL) for two weeks prior to breeding and until placenta were collected at embryonic age 12.5. A portion of the placenta was fixed for histology, and the remainder was frozen for RNA isolation followed by RNAseq. Maternal OXY treatment reduced parietal trophoblast giant cell (pTGC) area and decreased the maternal blood vessel area within the labyrinth region. OXY exposure affected placental gene expression profiles in a sex dependent manner with female placenta showing up-regulation of many placental enriched genes, including Ceacam11, Ceacam14, Ceacam12, Ceacam13, Prl7b1, Prl2b1, Ctsq, and Tpbpa. In contrast, placenta of OXY exposed males had alteration of many ribosomal proteins. Weighted correlation network analysis revealed that in OXY female vs. CTL female comparison, select modules correlated with OXY-induced placental histological changes. Such associations were lacking in the male OXY vs. CTL male comparison. Results suggest OXY exposure alters placental histology. In response to OXY exposure, female placenta responds by upregulating placental enriched transcripts that are either unchanged or downregulated in male placenta. Such changes may shield female offspring from developmental origins of health and disease-based diseases. •Placental cells bathed by maternal blood are vulnerable to circulating opioids.•Maternal oxycodone (OXY) treatment reduces parietal trophoblast giant cells.•Maternal OXY exposure induces sex-specific placenta transcript changes.•Placental-specific transcripts are enriched in female placenta exposed to OXY.
doi_str_mv 10.1016/j.placenta.2020.08.006
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OXY exposure affected placental gene expression profiles in a sex dependent manner with female placenta showing up-regulation of many placental enriched genes, including Ceacam11, Ceacam14, Ceacam12, Ceacam13, Prl7b1, Prl2b1, Ctsq, and Tpbpa. In contrast, placenta of OXY exposed males had alteration of many ribosomal proteins. Weighted correlation network analysis revealed that in OXY female vs. CTL female comparison, select modules correlated with OXY-induced placental histological changes. Such associations were lacking in the male OXY vs. CTL male comparison. Results suggest OXY exposure alters placental histology. In response to OXY exposure, female placenta responds by upregulating placental enriched transcripts that are either unchanged or downregulated in male placenta. 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OXY exposure affected placental gene expression profiles in a sex dependent manner with female placenta showing up-regulation of many placental enriched genes, including Ceacam11, Ceacam14, Ceacam12, Ceacam13, Prl7b1, Prl2b1, Ctsq, and Tpbpa. In contrast, placenta of OXY exposed males had alteration of many ribosomal proteins. Weighted correlation network analysis revealed that in OXY female vs. CTL female comparison, select modules correlated with OXY-induced placental histological changes. Such associations were lacking in the male OXY vs. CTL male comparison. Results suggest OXY exposure alters placental histology. In response to OXY exposure, female placenta responds by upregulating placental enriched transcripts that are either unchanged or downregulated in male placenta. 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subjects Analgesic
Analgesics, Opioid - adverse effects
Animals
DOHaD
Drug abuse
Female
Male
Mice
Opioid
Oxycodone - adverse effects
Placenta - drug effects
Placenta - metabolism
Placental lactogens
Pregnancy
Pregnancy Rate
Reproduction
Sex Ratio
Sexual dimorphism
Transcriptome - drug effects
Trophoblast
Trophoblast giant cells
title Maternal oxycodone treatment causes pathophysiological changes in the mouse placenta
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