Enrichment and physiological characterization of a novel comammox Nitrospira indicates ammonium inhibition of complete nitrification

• Published in: The ISME Journal 2021-04, Vol.15 (4), p.1010-1024
• Main Authors: Sakoula, Dimitra, Koch, Hanna, Frank, Jeroen, Jetten, Mike S. M., van Kessel, Maartje A. H. J., Lücker, Sebastian
• Format: Article
• Language: English
• Subjects: 14/19; 14/32; 14/63; 38/23; 45/22; 631/326/171; 631/326/41/1969; 631/326/41/2530; Affinity; Ammonia; Ammonium; Ammonium Compounds; Bacteria - genetics; Biomedical and Life Sciences; Cell culture; Ecology; Ecosystem; Enrichment media; Evolutionary Biology; Gene sequencing; Genomes; Life Sciences; Metagenomics; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Nitrification; Nitrites; Nitrogen dioxide; Nitrospira; Oligotrophic environments; Oxidation; Oxidation-Reduction; Physiology
• ISSN: 1751-7362; 1751-7370
• DOI: 10.1038/s41396-020-00827-4

The recent discovery of bacteria within the genus Nitrospira capable of complete ammonia oxidation (comammox) demonstrated that the sequential oxidation of ammonia to nitrate via nitrite can also be performed within a single bacterial cell. Although comammox Nitrospira exhibit a wide distribution in natural and engineered ecosystems, information on their physiological properties is scarce due to the limited number of cultured representatives. Additionally, most available genomic information is derived from metagenomic sequencing and high-quality genomes of Nitrospira in general are limited. In this study, we obtained a high (90%) enrichment of a novel comammox species, tentatively named “ Candidatus Nitrospira kreftii”, and performed a detailed genomic and physiological characterization. The complete genome of “ Ca . N. kreftii” allowed reconstruction of its basic metabolic traits. Similar to Nitrospira inopinata , the enrichment culture exhibited a very high ammonia affinity (K m(app)_NH3  ≈ 0.040 ± 0.01 µM), but a higher nitrite affinity (K m(app)_NO2 - = 12.5 ± 4.0 µM), indicating an adaptation to highly oligotrophic environments. Furthermore, we observed partial inhibition of ammonia oxidation at ammonium concentrations as low as 25 µM. This inhibition of “ Ca . N. kreftii” indicates that differences in ammonium tolerance rather than affinity could potentially be a niche determining factor for different comammox Nitrospira .