Schizosaccharomyces pombe KAT5 contributes to resection and repair of a DNA double-strand break

Abstract Chromatin remodeling is essential for effective repair of a DNA double-strand break (DSB). KAT5 (Schizosaccharomyces pombe Mst1, human TIP60) is a MYST family histone acetyltransferase conserved from yeast to humans that coordinates various DNA damage response activities at a DNA DSB, inclu...

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Bibliographic Details
Published in:Genetics (Austin) 2021-05, Vol.218 (1)
Main Authors: Li, Tingting, Petreaca, Ruben C, Forsburg, Susan L
Format: Article
Language:English
Subjects:
Chromatin - metabolism
Chromatin Assembly and Disassembly
Chromatin remodeling
Chromosomes, Fungal - metabolism
Damage
Deoxyribonucleic acid
DNA
DNA Breaks, Double-Stranded
DNA damage
DNA Repair
DNA, Fungal - genetics
Double-strand break repair
Endodeoxyribonucleases - genetics
Genetics
Histone acetyltransferase
Histone Acetyltransferases - genetics
Histone Acetyltransferases - metabolism
Histones
Histones - metabolism
Homologous Recombination
Homology
Investigation
Lysine Acetyltransferase 5 - genetics
Lysine Acetyltransferase 5 - metabolism
Mutation
Phenotypes
Repair
Schizosaccharomyces - genetics
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins - genetics
Schizosaccharomyces pombe Proteins - metabolism
Yeast
Yeasts
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Summary:Abstract Chromatin remodeling is essential for effective repair of a DNA double-strand break (DSB). KAT5 (Schizosaccharomyces pombe Mst1, human TIP60) is a MYST family histone acetyltransferase conserved from yeast to humans that coordinates various DNA damage response activities at a DNA DSB, including histone remodeling and activation of the DNA damage checkpoint. In S. pombe, mutations in mst1+ causes sensitivity to DNA damaging drugs. Here we show that Mst1 is recruited to DSBs. Mutation of mst1+ disrupts recruitment of repair proteins and delays resection. These defects are partially rescued by deletion of pku70, which has been previously shown to antagonize repair by homologous recombination (HR). These phenotypes of mst1 are similar to pht1-4KR, a nonacetylatable form of histone variant H2A.Z, which has been proposed to affect resection. Our data suggest that Mst1 functions to direct repair of DSBs toward HR pathways by modulating resection at the DSB.
ISSN:1943-2631
0016-6731
1943-2631
DOI:10.1093/genetics/iyab042