Ischemia-Reperfusion Injuries Assessment during Pancreas Preservation

Maintaining organ viability between donation and transplantation is of critical importance for optimal graft function and survival. To date in pancreas transplantation, static cold storage (SCS) is the most widely practiced method of organ preservation. The first experiments in ex vivo perfusion of...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-05, Vol.22 (10), p.5172
Main Authors: Prudhomme, Thomas, Mulvey, John F., Young, Liam A. J., Mesnard, Benoit, Lo Faro, Maria Letizia, Ogbemudia, Ann Etohan, Dengu, Fungai, Friend, Peter J., Ploeg, Rutger, Hunter, James P., Branchereau, Julien
Format: Article
Language:English
Subjects:
Abdomen
Animals
Biopsy
Caspase
Cell activation
Cell death
Cold storage
Cryopreservation
Edema
Endothelium
Hemorrhage
Histology
Humans
Ischemia
Kidneys
Life Sciences
Liver
Magnetic resonance imaging
Metabolites
Mitochondria
Organ Preservation
Oxygen consumption
Oxygen tension
Pancreas
Pancreas Transplantation
Perfusion
Reperfusion
Reperfusion Injury
Review
Small intestine
Tissue Survival
Transplants & implants
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Summary:Maintaining organ viability between donation and transplantation is of critical importance for optimal graft function and survival. To date in pancreas transplantation, static cold storage (SCS) is the most widely practiced method of organ preservation. The first experiments in ex vivo perfusion of the pancreas were performed at the beginning of the 20th century. These perfusions led to organ oedema, hemorrhage, and venous congestion after revascularization. Despite these early hurdles, a number of factors now favor the use of perfusion during preservation: the encouraging results of HMP in kidney transplantation, the development of new perfusion solutions, and the development of organ perfusion machines for the lung, heart, kidneys and liver. This has led to a resurgence of research in machine perfusion for whole organ pancreas preservation. This review highlights the ischemia-reperfusion injuries assessment during ex vivo pancreas perfusion, both for assessment in pre-clinical experimental models as well for future use in the clinic. We evaluated perfusion dynamics, oedema assessment, especially by impedance analysis and MRI, whole organ oxygen consumption, tissue oxygen tension, metabolite concentrations in tissue and perfusate, mitochondrial respiration, cell death, especially by histology, total cell free DNA, caspase activation, and exocrine and endocrine assessment.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22105172