Molecule-Resolved Visualization of Particulate Matter on Human Skin Using Multimodal Nonlinear Optical Imaging

Precise measurement of particulate matter (PM) on skin is important for managing and preventing PM-related skin diseases. This study aims to directly visualize the deposition and penetration of PM into human skin using a multimodal nonlinear optical (MNLO) imaging system. We successfully obtained PM...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2021-05, Vol.22 (10), p.5199
Main Authors: Lee, Eun-Soo, Kim, Suho, Lee, Sang-Won, Jung, Jinsang, Lee, Sung Hoon, Kim, Hyoung-June, Hong, Yong Deog, Park, Won Seok, Lee, Tae Geol, Jo, Dong-Gyu, Kim, Se-Hwa
Format: Article
Language:English
Subjects:
Biopsy
Collagen
Cytokines
Deposition
Elastin
Imaging
Inflammation
Investigations
Keratin
Nonlinear optics
Particulate emissions
Particulate matter
Skin
Skin diseases
Spatial distribution
Spectrum analysis
Three dimensional imaging
Visualization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Precise measurement of particulate matter (PM) on skin is important for managing and preventing PM-related skin diseases. This study aims to directly visualize the deposition and penetration of PM into human skin using a multimodal nonlinear optical (MNLO) imaging system. We successfully obtained PM particle signals by merging two different sources, C–C vibrational frequency and autofluorescence, while simultaneously visualizing the anatomical features of the skin via keratin, collagen, and elastin. As a result, we found morphologically dependent PM deposition, as well as increased deposition following disruption of the skin barrier via tape-stripping. Furthermore, PM penetrated more and deeper into the skin with an increase in the number of tape-strippings, causing a significant increase in the secretion of pro-inflammatory cytokines. Our results suggest that MNLO imaging could be a useful technique for visualizing and quantifying the spatial distribution of PM in ex vivo human skin tissues.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22105199