Oxidative Stress-Dependent Synergistic Antiproliferation, Apoptosis, and DNA Damage of Ultraviolet-C and Coral-Derived Sinularin Combined Treatment for Oral Cancer Cells

Combined treatment is increasingly used to improve cancer therapy. Non-ionizing radiation ultraviolet-C (UVC) and sinularin, a coral Sinularia flexibilis-derived cembranolide, were separately reported to provide an antiproliferation function to some kinds of cancer cells. However, an antiproliferati...

Full description

Saved in:
Bibliographic Details
Published in:Cancers 2021-05, Vol.13 (10), p.2450
Main Authors: Peng, Sheng-Yao, Tang, Jen-Yang, Li, Ruei-Nian, Huang, Hurng-Wern, Wu, Chang-Yi, Chiu, Chien-Chih, Chang, Fang-Rong, Zhang, Hong-Wei, Lee, Yun-Jou, Sheu, Jyh-Horng, Chang, Hsueh-Wei
Format: Article
Language:English
Subjects:
Acetylcysteine
Annexin V
Antioxidants
Apoptosis
Cancer therapies
Caspase-3
Cell cycle
Cell proliferation
Cell viability
Cytotoxicity
Deoxyguanosine
Deoxyribonucleic acid
DNA
DNA damage
Flow cytometry
Ionizing radiation
Membrane potential
Mitochondrial DNA
Natural products
Oral cancer
Oxidative stress
Radiation
Reactive oxygen species
Ribose
Side effects
Superoxide
Tumor cell lines
Ultraviolet radiation
Western blotting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Combined treatment is increasingly used to improve cancer therapy. Non-ionizing radiation ultraviolet-C (UVC) and sinularin, a coral Sinularia flexibilis-derived cembranolide, were separately reported to provide an antiproliferation function to some kinds of cancer cells. However, an antiproliferation function using the combined treatment of UVC/sinularin has not been investigated as yet. This study aimed to examine the combined antiproliferation function and explore the combination of UVC/sinularin in oral cancer cells compared to normal oral cells. Regarding cell viability, UVC/sinularin displays the synergistic and selective killing of two oral cancer cell lines, but remains non-effective for normal oral cell lines compared to treatments in terms of MTS and ATP assays. In tests using the flow cytometry, luminescence, and Western blotting methods, UVC/sinularin-treated oral cancer cells exhibited higher reactive oxygen species production, mitochondrial superoxide generation, mitochondrial membrane potential destruction, annexin V, pan-caspase, caspase 3/7, and cleaved-poly (ADP-ribose) polymerase expressions than that in normal oral cells. Accordingly, oxidative stress and apoptosis are highly induced in a combined UVC/sinularin treatment. Moreover, UVC/sinularin treatment provides higher G2/M arrest and γH2AX/8-hydroxyl-2′deoxyguanosine-detected DNA damages in oral cancer cells than in the separate treatments. A pretreatment can revert all of these changes of UVC/sinularin treatment with the antioxidant N-acetylcysteine. Taken together, UVC/sinularin acting upon oral cancer cells exhibits a synergistic and selective antiproliferation ability involving oxidative stress-dependent apoptosis and cellular DNA damage with low toxic side effects on normal oral cells.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers13102450