Biofunctional Janus particles promote phagocytosis of tumor cells by macrophages

Herein, a versatile strategy for the construction of biofunctional Janus particles (JPs) through the combination of Pickering emulsion and copper-free click chemistry is developed for the study of particle-mediated cell-cell interactions. A variety of biomolecules including bovine serum albumin (BSA...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2020-05, Vol.11 (2), p.5323-5327
Main Authors: Zhang, Ya-Ru, Luo, Jia-Qi, Li, Jia-Xian, Huang, Qiu-Yue, Shi, Xiao-Xiao, Huang, Yong-Cong, Leong, Kam W, He, Wei-ling, Du, Jin-Zhi
Format: Article
Language:English
Subjects:
Alpha iron
Antibodies
Biomolecules
Chemical synthesis
Chemistry
Ferritin
Macrophages
Nanoparticles
Phagocytosis
Serum albumin
Transferrin
Tumors
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Summary:Herein, a versatile strategy for the construction of biofunctional Janus particles (JPs) through the combination of Pickering emulsion and copper-free click chemistry is developed for the study of particle-mediated cell-cell interactions. A variety of biomolecules including bovine serum albumin (BSA), ferritin, transferrin (Tf), and anti-signal regulatory protein alpha antibodies (aSIRPα), etc. , can be incorporated into the Janus platform in a spatially defined manner. JPs consisting of Tf and aSIRPα (Tf-SPA1-aSIRPα JPs) demonstrate a significantly improved binding affinity to either macrophages or tumor cells compared to their uniformly modified counterparts. More importantly, Tf-SPA1-aSIRPα JPs mediate more efficient phagocytosis of tumor cells by macrophages as revealed by real-time high-content confocal microscopy. This study demonstrates the potential advantages of JPs in mediating cell-cell interactions and may contribute to the emerging cancer immunotherapy. A versatile Janus particle platform modified with biological ligands can facilitate tumor cell phagocytosis by macrophages for promising cancer immunotherapy.
ISSN:2041-6520
2041-6539
DOI:10.1039/d0sc01146k